Prohibitin 2 Antibody - BSA Free

Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NB100-1807] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- REA used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunoprecipitation: Prohibitin 2 Antibody [NB100-1807] - Detection of Human REA by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg/IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-REA antibodies NB100-1807, another REA Ab, NB100-1808, and NB100-1809 each used for IP at 3 mcg/mg lysate. Immunoprecipitated REA was blotted using BL1706 (upper blot) or BL1707 (lower blot). Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower

• Reactivity: Hu, Mu, Pm-Or
• Applications: IHC, IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Prohibitin 2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Prohibitin 2 Antibody - BSA Free (NB100-1807) is a polyclonal antibody validated for use in IHC and IP. Anti-Prohibitin 2 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Repressor of Estrogen Activity (Prohibitin 2) using the numbering given in entry NP_009204.1 (GeneID 11331).
• Predicted Species: Mouse (100%), Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PHB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000- 1:5000
  • Immunohistochemistry-Paraffin 1:1000- 1:5000
  • Immunoprecipitation 1-4 ug/mg of lysate
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Western Blot Not recommended.
• Control:

  Prohibitin 2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Prohibitin 2 Antibody - BSA Free

• BAP
• Bap37
• BCAP37
• B-cell associated protein
• D-Prohibitin
• MGC117268
• p22
• PHB2
• PNAS-141
• Prohibitin 2
• prohibitin-2
• REA
• REAB-cell receptor-associated protein BAP37
• Repressor of estrogen receptor activity

Background

REA (repressor of estrogen receptor activity), also known as prohibitin 2, has been shown to exhibit a variety of functions that includes mitochondrial chaperone activity, cell cycle regulation, and transcriptional regulation. REA localizes to the mitochondria and associates with mitochondrial complex I where it may play a role in mitochondrial metabolism. REA interacts with pRb to inhibit E2F-mediated transcription as well as the estrogen receptor to repress estrogen-dependent transcription via recruitment of histone deacetylases. Alternate names for REA include B-cell receptor-associated protein BAP37, D-prohibitin, PHB2, BAP, p22, BCAP37, PNAS-141, and MGC117268.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Prohibitin 2 Antibody (NB100-1807)(3)

Publications using NB100-1807

• Liu D, Lin Y, Kang T et al. Mitochondrial dysfunction and adipogenic reduction by prohibitin silencing in 3T3-L1 cells. PLoS One 2012-01-01 [PMID: 22479600]
• Strub GM, Paillard M, Liang J et al. Sphingosine-1-phosphate produced by sphingosine kinase 2 in mitochondria interacts with prohibitin 2 to regulate complex IV assembly and respiration. FASEB J 2011-02-01 [PMID: 20959514]
• He B, Feng Q, Mukherjee A et al. A repressive role for prohibitin in estrogen signaling. Mol Endocrinol 2008-02-01 [PMID: 17932104]

Bioinformatics

• Gene Symbol: PHB2
• Entrez:
  • 11331(Human)
• Uniprot:
  • Q99623()