Human Progesterone R/NR3C3 ELISA Kit (Chemiluminescence) Summary
Specificity |
This kit recognizes Human PGR in samples. No significant cross-reactivity or interference between Human PGR and analogues was observed. |
Standard Curve Range |
62.50-4000 pg/mL |
Sensitivity |
37.50 pg/mL |
Assay Type |
Sandwich-CLIA |
Inter-Assay |
CV% < 8.7% |
Intra-Assay |
CV% < 9.15% |
Spike Recovery |
88-114% |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Chemiluminescence) |
Gene |
PGR |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Biotinylated Detection Ab Diluent
- Certificate of Analysis
- Concentrated Biotinylated Detection Ab (100×)
- Concentrated HRP Conjugate (100×)
- Concentrated Wash Buffer (25×)
- HRP Conjugate Diluent
- Micro CLIA Plate(Dismountable)
- Plate Sealer
- Product Description
- Reference Standard & Sample Diluent
- Reference Standard
- Substrate Reagent A
- Substrate Reagent B
|
Alternate Names for Human Progesterone R/NR3C3 ELISA Kit (Chemiluminescence)
Background
The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Progesterone R/NR3C3 ELISA Kit (NBP2-68091). (Showing 1 - 1 of 1 FAQ).
-
I am interested in getting antibodies for PR (progesterone receptor) that would work in flow cytometry. Could you recommend some choices?
- We do not currently have any antibodies directed towards the progesterone receptor that have been validated in flow cytometry, but any of our antibodies that work in ICC should work just fine in flow as they are essentially the same application. Please see this link for our Progesterone Receptor antibodies validated in ICC.
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