Progesterone R/NR3C3 Antibody (5D10)

Immunocytochemistry/Immunofluorescence: Progesterone R/NR3C3 Antibody (5D10) [H00005241-M07] - Analysis of monoclonal antibody to PGR on MCF-7 cell. Antibody concentration 20 ug/ml

Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (5D10) [H00005241-M07] - Analysis of monoclonal antibody to PGR on formalin-fixed paraffin-embedded human breast cancer. Antibody concentration 3 ug/ml

ELISA: Progesterone R/NR3C3 Antibody (5D10) [H00005241-M07] - Detection limit for recombinant GST tagged PGR is approximately 0.03ng/ml as a capture antibody.

Product Discontinued

Progesterone R/NR3C3 Antibody (5D10) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PGR (NP_000917, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTELKAKGPRAPHVAGGPPSPEVGSPLLCRPAAGPFPGSQTSDTLPEVSAIPISLDGLLFPRPCQGQDPSDEKTQDQQSLSDVEGAYSRAEATRGAGGSSSSPPEKDSGL
• Localization: Nuclear
• Specificity: PGR - progesterone receptor
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PGR
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA. Use in Immunohistochemistry reported in scientific literature (PMID: 22553415).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Progesterone R/NR3C3 Antibody (5D10)

• NR3C3
• NR3C3PRNuclear receptor subfamily 3 group C member 3
• PGR
• Progesterone R
• progesterone receptor
• ProgesteroneR

Background

The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Progesterone R/NR3C3 Antibody (H00005241-M07)(2)

Publications using H00005241-M07

• Liu LY, Chang LY, Kuo WH et al. In Silico Prediction for Regulation of Transcription Factors onTheir Shared Target Genes Indicates Relevant Clinical Implications in a Breast Cancer Population. Cancer Inform. 2012-01-01 [PMID: 22553415] (IF/IHC)
• Liu LY, Chang LY, Kuo WH et al. Major Functional Transcriptome of an Inferred Center Regulator of an ER(-) Breast Cancer Model System. Cancer Inform. 2012-04-19 [PMID: 22553414]

FAQs for Progesterone R/NR3C3 Antibody (H00005241-M07). (Showing 1 - 1 of 1 FAQs).

1. I am interested in getting antibodies for PR (progesterone receptor) that would work in flow cytometry. Could you recommend some choices?
   • We do not currently have any antibodies directed towards the progesterone receptor that have been validated in flow cytometry, but any of our antibodies that work in ICC should work just fine in flow as they are essentially the same application. Please see this link for our Progesterone Receptor antibodies validated in ICC.

Bioinformatics

• Gene Symbol: PGR
• Entrez:
  • 5241(Human)
• OMIM:
  • 607311(Human)
• Uniprot:
  • NP_000917()