Proenkephalin Antibody

Immunohistochemistry-Frozen: Proenkephalin Antibody [NBP1-20963] - Staining of PFA-perfused cryosection of Rat Striatum. HRP-staining with Ni-DAB after Biotin-SP-anti-goat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.

Immunohistochemistry-Frozen: Proenkephalin Antibody [NBP1-20963] - Staining of PFA-perfused cryosection of Mouse Stria terminalis. HRP-staining with Ni-DAB after Biotin-SP-anti-goat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.

• Reactivity: Mu, Rt
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Proenkephalin Antibody Summary

• Immunogen: Peptide with sequence C-YKDSSKQDESH corresponding to internal region according to Uniprot Mouse NP_001002927.1.
• Epitope: C terminus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PENK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.02 - 0.05 ug/ml
  • Immunohistochemistry-Frozen 0.02-0.05 ug/ml
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments gave an approx. 40 kDa band in rat brain lysates after 0.2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.8 kDa band according to mouse NP_001002927.1 and 30.9 kDa band according to rat NP_058835.1. The 40 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-Fr: Rat brain (striatum) shows staining of dense enkephalinergic axon plexus. Mouse brain (stria terminalis) shows staining of a dense enkephalinergic fibre network in the bed nucleus.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Proenkephalin Antibody

• enkephalin A
• preproenkephalin
• proenkephalin
• proenkephalin-A

Background

Enkephalins are small peptides derived from large precursers (pro-enkephalin A and B) containing multiple enkephalin copies. They are the most abundant opioid peptides in the body and are widely distributed in the brain and the peripheral nervous system and occur also in the adrenal medulla. Several types of neuroendocrine tumors, including pheochromocytomas, neuroblastomas and bronchial and gastrointestinal endocrine tumors, produce enkephalin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Proenkephalin Antibody (NBP1-20963)(2)

Publications using NBP1-20963

• Kim H, Kim D, Ha C Et al. Transcriptional Regulatory Role of NELL2 in Preproenkephalin Gene Expression Mol Cells 2022-08-11 [PMID: 35950455]
• Schiltz CA, Bremer QZ, Landry CF, Kelley AE. Food-associated cues alter forebrain functional connectivity as assessed with immediate early gene and proenkephalin expression. BMC Biol 2007-04-26 [PMID: 17462082]

Bioinformatics

• Gene Symbol: PENK
• Entrez:
  • 110182(Mouse)
  • 29237(Rat)
• Uniprot:
  • NP_001002927.1(Mouse)