Recombinant Human PRL-3/PTP4A3 His Protein

SDS-Page: Recombinant Human PRL-3/PTP4A3 Protein [NBP1-30246] - PRL-3, 21.6 kDa (193aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human PRL-3/PTP4A3 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-173 of Human PRL-3/PTP4A3
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MARMNRPAPV EVSYKHMRFL ITHNPTNATL STFIEDLKKY GATTVVRVCE VTYDKTPLEK DGITVVDWPF DDGAPPPGKV VEDWLSLVKA KFCEAPGSCV AVHCVAGLGR APVLVALALI ESGMKYEDAI QFIRQKRRGA INSKQLTYLE KYRPKQRLRF KDPHTHKTRC CVM

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PTP4A3
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 21.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 2 mM EDTA, 1 mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human PRL-3/PTP4A3 His Protein

• PRL3
• PRL-3
• PRL3EC 3.1.3.48
• PRL-3potentially prenylated protein tyrosine phosphatase
• PRL-Rprotein tyrosine phosphatase type IVA 3
• protein tyrosine phosphatase type IVA, member 3
• Protein-tyrosine phosphatase 4a3
• Protein-tyrosine phosphatase of regenerating liver 3
• PTP4A3

Background

Protein tyrosine phosphatase type IVA 3 (PRL-3), as known as PTP4A3, belongs to a small class of prenylated protein tyrosine phosphatases (PTPs) that remove phosphate modifications from tyrosine residues on proteins. This protein enhances cell proliferation, cell motility and invasive activity. High levels of PRL-3 expression are associated with tumorigenesis and metastasis, thus it is overexpressed in metastatic colorectal, ovarian, liver and skin cancers. Recombinant human PRL-3, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PTP4A3