PRICKLE1 Antibody - BSA Free

Western Blot: PRICKLE1 Antibody - BSA Free [NBP3-46898] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (PRICKLE1 antibody) at dilution of 1:1000

Immunoprecipitation: PRICKLE1 Antibody - BSA Free [NBP3-46898] - IP Result of anti-PRICKLE1 (IP:, 3ug; Detection: 1:1000) with mouse brain tissue lysate 7000ug.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PRICKLE1 Antibody - BSA Free Summary

• Immunogen: prickle homolog 1(Drosophila). (Uniprot# Q96MT3)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRICKLE1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation 1:200-1:2000
  • Western Blot 1:500-1:2000
• Application Notes: Theoretical Molecular Weight is 94 kDa, 55-60 kDa

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PRICKLE1 Antibody - BSA Free

• EPM1BMGC138902
• FLJ31627
• FLJ31937
• prickle homolog 1 (Drosophila)
• prickle-like 1 (Drosophila)
• prickle-like 1
• prickle-like protein 1
• REST (RE-1 silencing transcription factor)/NRSF (neuron-restrictive silencerfactor)-interacting LIM domain protein
• REST/NRSF-interacting LIM domain protein 1
• RILPMGC138903

Background

PRICKLE1 has 831 amino acids and is approximately 94kDa. PRICKLE1 localizes to the nuclear membrane and is involved in the movement of REST, a transcription repressor. PRICKLE1 is implicated in pathways that regulate neural tube development and gastrulation. PRICKLE1 may also be a nuclear receptor that negatively regulates the Wnt/beta-catenin signaling pathway. Current research on PRICKLE1 is being performed in relation to several diseases and disorders including neural tube defects and progressive myoclonic epilepsy type 1B. PRICKLE1 has also been shown to interact with Dishevelled 1, Dishevelled 2, Dishevelled 3, VIP, and SMURF2 in the Wnt signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRICKLE1