PRDM9 Antibody (RAB-C370) [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IP, ChIP, MS
• Clone: RAB-C370
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: CoraFluor 1

PRDM9 Antibody (RAB-C370) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained by recombinant antibody (rAb) phage display recognizing PRDM9 protein under non-denaturing conditions.
• Epitope: This antibody binds to a folded domain, amino acids 195-385.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRDM9
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Mass Spectrometry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for PRDM9 Antibody (RAB-C370) [CoraFluor™ 1]

• EC 2.1.1.-
• EC 2.1.1.354
• EC 2.1.1.359
• EC 2.1.1.361
• EC 2.1.1.362
• EC 2.1.1.43
• histone-lysine N-methyltransferase PRDM9
• minisatellite binding protein 3 (115kD)
• minisatellite binding protein 3, 115kDa
• PFM6
• PFM6MEISETZ
• PR domain containing 9
• PR domain zinc finger protein 9
• PR domain-containing protein 9
• PRDM9
• PRMD9
• ZNF899

Background

The PR domain is a protein-protein interaction module of about 100 amino acids. PR domain-containing proteins, such as PRDM9, are often involved in transcriptional regulation (Jiang and Huang, 2000 (PubMed 10668202)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRDM9