PRAS40 [p Thr356] Antibody - Azide Free

Western Blot: PRAS40 [p Thr356] Antibody [NBP2-60783] - Western blot of Drospohila lysate showing specific immunolabeling of the ~60 kDa PRAS40 protein phosphorylated at Thr356 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (Lambda-Ptase, 1200 units for 30 minutes).

• Reactivity: Dr
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

PRAS40 [p Thr356] Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit PRAS40 [p Thr356] Antibody - Azide Free (NBP2-60783) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr356 conjugated to KLH
• Modification: p Thr356
• Specificity: Specific for endogenous levels of the ~60 kDa PRAS40 protein phosphorylated at Thr356. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AKT1S1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for PRAS40 [p Thr356] Antibody - Azide Free

• 40 kDa
• AKT1 substrate 1 (proline-rich)
• AKT1S1
• Lobe
• MGC2865
• PRAS40
• proline-rich AKT1 substrate 1,40 kDa proline-rich AKT substrate

Background

Proline Rich Akt Substrate (PRAS40) is a 40 kDa substrate of Akt. Akt and PRAS40 can be found in the phosphoinositide 3 kinase (PI3K) pathway, which plays a role in glucose uptake, cell growth, and apoptosis inhibition. PRAS40 is a 14-3-3 binding protein that reacts with insulin, but whose precise function is not yet known. It may bind SH3 and WW domain containing proteins resulting in a change of function. Activated Akt phosphorylates PRAS40 on threonine 246. Mutation of PRAS40 threonine 246 has been shown to be apoptotic.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AKT1S1