PPP2R5B Antibody

Western Blot: PPP2R5B Antibody [NB100-850] - Antibody at 2 ug/ml. Human cerebellum lysate (35 ug protein in RIPA buffer). Detection by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PPP2R5B Antibody Summary

• Immunogen: Peptide with sequence C-QRLTPQVAASGGQS corresponding to C-Terminus according to NP_006235.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PPP2R5B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 2 - 3 ug/ml
• Application Notes: WB: Approx. 55 kDa band observed in human brain (cerebellum) lysates (calculated MW of 57.4 kDa band according to NP_006235.1). Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.
• Control:

  PPP2R5B Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PPP2R5B Antibody

• B56B
• FLJ35411
• PP2A B subunit isoform B56-beta
• PP2A B subunit isoform B'-beta
• PP2A B subunit isoform PR61-beta
• PP2A B subunit isoform R5-beta
• PR61B
• protein phosphatase 2, regulatory subunit B (B56), beta isoform
• protein phosphatase 2, regulatory subunit B', beta isoform
• protein phosphatase 2, regulatory subunit B', beta
• serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, betaisoform
• serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform

Background

The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B56 subfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PPP2R5B Antibody (NB100-850)(2)

Publications using NB100-850

• Cristobal I, Garcia-Orti L, Cirauqui C et al. PP2A impaired activity is a common event in acute myeloid leukemia and its activation by forskolin has a potent anti-leukemic effect Leukemia 2011-04-01 [PMID: 21233840] (WB, Human)
• McCright B, Virshup DM. Identification of a new family of protein phosphatase 2A regulatory subunits. J Biol Chem 1995-11-03 [PMID: 7592815]

Bioinformatics

• Gene Symbol: PPP2R5B
• Entrez:
  • 5526(Human)
  • 225849(Mouse)
• Uniprot:
  • NP_006235.(Human)