PPP2R2C Antibody (6D1) - Azide and BSA Free

Western Blot: PPP2R2C Antibody (6D1) [H00005522-M01] - Analysis of PPP2R2C expression in transfected 293T cell line by PPP2R2C monoclonal antibody (M01), clone 6D1.Lane 1: PPP2R2C transfected lysate(49.5 KDa).Lane 2: Non-transfected lysate.

Western Blot: PPP2R2C Antibody (6D1) [H00005522-M01] - PPP2R2C monoclonal antibody (M01), clone 6D1 Analysis of PPP2R2C expression in HeLa.

ELISA: PPP2R2C Antibody (6D1) [H00005522-M01] - Detection limit for recombinant GST tagged PPP2R2C is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 6D1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PPP2R2C Antibody (6D1) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PPP2R2C (NP_065149, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGEDTDTRKINHSFLRDHSYVTEADIISTVEFNHTGELLATGDKGGRVVIFQREPESKNAPHSQGEYDVYSTFQSHEPEFDYLKSLEIEEKINKIKWLPQQNAAHSLLST
• Localization: Cytoplasmic
• Specificity: PPP2R2C - protein phosphatase 2 (formerly 2A), regulatory subunit B (PR 52), gamma isoform
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPP2R2C
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PPP2R2C Antibody (6D1) - Azide and BSA Free

• B55-GAMMA
• IMYPNO1
• phosphoprotein phosphatase 2A BR gamma regulatory chain
• PP2A subunit B isoform B55-gamma
• PP2A subunit B isoform gamma
• PP2A subunit B isoform PR55-gamma
• PP2A subunit B isoform R2-gamma
• PP2A, subunit B, B55-gamma isoform
• PP2A, subunit B, B-gamma isoform
• PP2A, subunit B, PR55-gamma isoform
• PP2A, subunit B, R2-gamma isoform
• PR55G
• protein phosphatase 2 (formerly 2A), regulatory subunit B (PR 52), gammaisoform
• protein phosphatase 2 (formerly 2A), regulatory subunit B, gamma isoform
• protein phosphatase 2
• protein phosphatase 2, regulatory subunit B, gamma
• protein phosphatase 2A1 B gamma subunit
• serine/threonine protein phosphatase 2A, 55 KDA regulatory subunit B, gammaisoform
• serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B gammaisoform

Background

The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a gamma isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PPP2R2C Antibody (H00005522-M01)(2)

Publications using H00005522-M01

• Bluemn EG, Spencer ES, Mecham B et al. PPP2R2C Loss Promotes Castration-Resistance and Is Associated with Increased Prostate Cancer-Specific Mortality. Mol Cancer Res. 2013-06-06 [PMID: 23493267]
• Elysia Sophia Spencer, Eric Gregory Bluemn, Richard Johnston et al. Association of decreased expression of protein phosphatase 2A subunit PR55{gamma} (PPP2R2C) with an increased risk of metastases and prostate cancer-specific mortality. Journal of Clinical Oncology 2012-05-20

Bioinformatics

• Gene Symbol: PPP2R2C
• Entrez:
  • 5522(Human)
• OMIM:
  • 605997(Human)