PPP2R2B Knockout HeLa Cell Lysate

Product Discontinued

PPP2R2B Knockout HeLa Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,-1 bp deletion in exon3
• Gene: PPP2R2B

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout HeLa Cell

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for PPP2R2B Knockout HeLa Cell Lysate

• B55BETA
• FLJ95686
• MGC24888
• PP2A subunit B isoform B55-beta
• PP2A subunit B isoform beta
• PP2A subunit B isoform PR55-beta
• PP2A subunit B isoform R2-beta
• PP2A, subunit B, B-beta isoform
• PP2AB55BETA
• PP2ABBETA
• PP2APR55B
• PP2APR55BETA
• PR2AB55BETA
• PR2ABBETA
• PR2APR55BETA
• PR52B
• PR55BETA
• PR55-BETA
• protein phosphatase 2 (formerly 2A), regulatory subunit B (PR 52), beta isoform
• protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform
• protein phosphatase 2, regulatory subunit B, beta
• SCA12
• serine/threonine protein phosphatase 2A, 55 kDa regulatory subunit B, betaisoform
• serine/threonine protein phosphatase 2A, neuronal isoform
• serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B betaisoform
• spinocerebellar ataxia 12

Background

The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B55 subfamily. Defects in this gene cause autosomal dominant spinocerebellar ataxia 12 (SCA12), a disease caused by degeneration of the cerebellum, sometimes involving the brainstem and spinal cord, and in resulting in poor coordination of speech and body movements. Multiple alternatively spliced variants, which encode different isoforms, have been identified for this gene. The 5' UTR of some of these variants includes a CAG trinucleotide repeat sequence (7-28 copies) that can be expanded to 55-78 copies in cases of SCA12. [provided by RefSeq, Jul 2016]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: PPP2R2B