PPP2R1B Overexpression Lysate

Product Discontinued

PPP2R1B Overexpression Lysate Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_002707. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens PPP2R1B, mRNA.
• Gene: PPP2R1B

Applications/Dilutions

• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PPP2R1B Overexpression Lysate

• MGC26454
• PP2A subunit A isoform PR65-beta
• PP2A subunit A isoform R1-beta
• PP2A, subunit A, PR65-beta isoform
• PP2A, subunit A, R1-beta isoform
• PP2A-Abeta
• PR65B
• protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), beta isoform
• protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform
• protein phosphatase 2, regulatory subunit A, beta
• protein phosphatase 2, structural/regulatory subunit A, beta
• serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A betaisoform

Background

The PPP2R1B gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric co

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: PPP2R1B