PPP2R1A Antibody

Images

 
Western Blot: PPP2R1A Antibody [NBP2-19907] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: PPP2R1A Antibody [NBP2-19907] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: PPP2R1A Antibody [NBP2-19907] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.
Western Blot: PPP2R1A Antibody [NBP2-19907] - Sample (30 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Mk, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.96 mg/ml

Order Details

PPP2R1A Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PPP2R1A. The exact sequence is proprietary.
Predicted Species
Rat (100%), Porcine (100%), Xenopus (95%), Bovine (99%), Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPP2R1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:2500
  • Immunohistochemistry-Paraffin 1:100-1:2500
  • Immunoprecipitation
Application Notes
Immunoprecipitation Assay-dependent dilution
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%), Zebrafish (88%), Xenopus laevis (95%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.96 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PPP2R1A Antibody

  • Medium tumor antigen-associated 61 kDa protein
  • MGC786
  • PP2A subunit A isoform PR65-alpha
  • PP2A subunit A isoform R1-alpha
  • PP2AAALPHA
  • PP2A-Aalpha
  • PR65A
  • protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alphaisoform
  • protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform
  • protein phosphatase 2, regulatory subunit A, alpha
  • serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, alphaisoform
  • serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alphaisoform

Background

This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PPP2R1A
Uniprot