PPP1R7 Antibody (OTI4F9)

Western Blot: PPP1R7 Antibody (4F9) [NBP1-47924] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-PPP1R7 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunocytochemistry/Immunofluorescence: PPP1R7 Antibody (4F9) [NBP1-47924] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PPP1R7.

Immunohistochemistry-Paraffin: PPP1R7 Antibody (4F9) [NBP1-47924] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PPP1R7 mouse monoclonal antibody.

Western Blot: PPP1R7 Antibody (4F9) [NBP1-47924] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP1R7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP1R7.

Immunocytochemistry/Immunofluorescence: PPP1R7 Antibody (4F9) [NBP1-47924] - Immunofluorescent staining of HT29 cells using anti-PPP1R7 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PPP1R7 Antibody (4F9) [NBP1-47924] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PPP1R7 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ICC/IF, IHC
• Clone: OTI4F9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PPP1R7 Antibody (OTI4F9) Summary

• Immunogen: Full length human recombinant protein of human PPP1R7 (NP_002703) produced in HEK293T cell.
• Specificity: This antibody is specific for Homo sapiens protein phosphatase 1, regulatory (inhibitor) subunit 7 (PPP1R7).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPP1R7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
• Theoretical MW: 41.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PPP1R7 Antibody (OTI4F9)

• Protein phosphatase 1 regulatory subunit 22
• protein phosphatase 1 regulatory subunit 7
• protein phosphatase 1, regulatory (inhibitor) subunit 7
• protein phosphatase-1 regulatory subunit 7 alpha2
• protein phosphatase-1 regulatory subunit 7 beta1
• protein phosphatase-1 regulatory subunit 7 beta2
• regulatory subunit 7
• sds22

Background

Regulatory subunit of protein phosphatase 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP1R7
• Entrez:
  • 5510(Human)