PPIG Antibody

Western Blot: PPIG Antibody [NBP1-19117] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SRcyp antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SRcyp wasalso immunoprecipitated by rabbit anti-SRcyp antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30seconds (A) and 3 seconds (B).

Immunohistochemistry: PPIG Antibody [NBP1-19117] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- SRcyp used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PPIG Antibody Summary

• Description: Novus Biologicals Rabbit PPIG Antibody (NBP1-19117) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 450 and 500 of human SR-cyclophilin (Peptidyl-prolyl isomerase G) using the numbering given in entry NP_004783.2 (GeneID 9360).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPIG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PPIG Antibody

• CARS-cyclophilin
• CARS-CypPPIase G
• CASP10
• Clk-associating RS-cyclophilin
• Cyclophilin G
• CYP
• EC 5.2.1.8
• MGC133241
• peptidyl-prolyl cis-trans isomerase G
• peptidylprolyl isomerase G (cyclophilin G)
• peptidyl-prolyl isomerase G (cyclophilin G)
• Peptidyl-prolyl isomerase G
• Rotamase G
• SCAF10
• SR-cyclophilin
• SRCyp
• SR-cyp
• SR-related CTD-associated factor 10

Background

SR cyclophilin (SRcyp) is also known as peptidyl-prolyl cis-trans isomerase G. Cyclophilins are peptidyl-prolyl isomerases (PPIases) that accelerate the folding of proteins by catalyzing the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The function of cyclophilins is poorly understood, but their function has been linked to multiple cellular processes such as protein folding, trafficking, and chaperone activity. SRcyp is a member of the Moca family of nuclear cyclophilins and is phosphorylated in a cell cycle dependent manner. There is evidence that SRcyp may be involved in the regulation of gene expression and mRNA splicing. Alternative names for SRcyp include PPIase G, rotamase G, cyclophilin G, clk-associating RS-cyclophilin, CARS-cyclophilin, SR-cyp, CASP10, and PPIG.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPIG
• Entrez:
  • 9360(Human)
• Uniprot:
  • NP_004783.2(Human)