PPEF1 Antibody (1F6-1A5) - Azide and BSA Free

Western Blot: PPEF1 Antibody (1F6-1A5) [H00005475-M01] - Analysis of PPEF1 expression in transfected 293T cell line by PPEF1 monoclonal antibody (M01), clone 1F6-1A5.Lane 1: PPEF1 transfected lysate(75.8 KDa).Lane 2: Non-transfected lysate.

Western Blot: PPEF1 Antibody (1F6-1A5) [H00005475-M01] - Analysis of PPEF1 over-expressed 293 cell line, cotransfected with PPEF1 Validated Chimera RNAi ( Cat # H00005475-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PPEF1 monoclonal antibody (M01), clone 1F6-1A5 (Cat # H00005475-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

• Reactivity: Hu
• Applications: WB, ELISA, KD
• Clone: 1F6-1A5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PPEF1 Antibody (1F6-1A5) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PPEF1 (AAH36026, 1 a.a. ~ 653 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGCSSSSTKTRRSDTSLRAALIIQNWYRGYKARLKARQHYALTIFQSIEYADEQGQMQLSTFFSFMLENYTHIHKEELELRNQSLESEQDMRDRWDYVDSIDVPDSYNGPRLQFPLTCTDIDLLLEAFKEQQILHAHYVLEVLFETKKVLKQMPNFTHIQTSPSKEVTICGDLHGKLDDLFLIFYKNGLPSERNPYVFNGDFVDRGKNSIEILMILCVSFLVYPNDLHLNRGNHEDFMMNLRYGFTKEILHKYKLHGKRILQILEEFYAWLPIGTIVDNEILVIHGGISETTDLNLLHRVERNKMKSVLIPPTETNRDHDTDSKHNKVGVTFNAHGRIKTNGSPTEHLTEHEWEQIIDILWSDPRGKNGCFPNTCRGGGCYFGPDVTSKILNKYQLKMLIRSHECKPEGYEICHDGKVVTIFSASNYYEEGSNRGAYIKLCSGTTPRFFQYQVTKATCFQPLRQRVDTMENSAIKILRERVISRKSDLTRAFQLQDHRKSGKLSVSQWAFCMENILGLNLPWRSLSSNLVNIDQNGNVEYMSSFQNIRIEKPVQEAHSTLVETLYRYRSDLEIIFNAIDTDHSGLISVEEFRAMWKLFSSHYNVHIDDSQVNKLANIMDLNKDGSIDFNEFLKAFYVVHRYEDLMKPDVTNLG
• Specificity: PPEF1 - protein phosphatase, EF hand calcium-binding domain 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPEF1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA and RNAi Validation.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PPEF1 Antibody (1F6-1A5) - Azide and BSA Free

• EC 3.1.3.16
• PP7
• PPEF
• PPEF-1
• PPP7C
• PPP7CA
• protein phosphatase 7, catalytic subunit, alpha isozyme
• Protein phosphatase with EF calcium-binding domain
• protein phosphatase, EF-hand calcium binding domain 1
• protein phosphatase, serine/threonine type, with EF-hands
• serine/threonine protein phosphatase 7
• Serine/threonine-protein phosphatase 7
• serine/threonine-protein phosphatase with EF-hands 1

Background

This gene encodes a member of the serine/threonine protein phosphatase with EF-hand motif family. The protein contains a protein phosphatase catalytic domain, and at least two EF-hand calcium-binding motifs in its C terminus. Although its substrate(s) is unknown, the encoded protein has been suggested to play a role in specific sensory neuron function and/or development. This gene shares high sequence similarity with the Drosophila retinal degeneration C (rdgC) gene. Several alternatively spliced transcript variants, each encoding a distinct isoform, have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPEF1
• Entrez:
  • 5475(Human)
• OMIM:
  • 300109(Human)
• Uniprot:
  • AAH36026(Human)