PP5 Antibody - BSA Free

Western Blot: PP5 Antibody [NBP1-87240] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Immunocytochemistry/Immunofluorescence: PP5 Antibody [NBP1-87240] - Staining of human cell line U-251 MG shows localization to cytosol & vesicles. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: PP5 Antibody [NBP1-87240] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.

Western Blot: PP5 Antibody [NBP1-87240] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Immunohistochemistry-Paraffin: PP5 Antibody [NBP1-87240] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Immunohistochemistry-Paraffin: PP5 Antibody [NBP1-87240] - Staining of human colon shows weak cytoplasmicand moderate membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: PP5 Antibody [NBP1-87240] - Staining of human prostate shows weak cytoplasmic positivity in smooth muscle cells.

Immunohistochemistry-Paraffin: PP5 Antibody [NBP1-87240] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in a subset of neurons.

• Reactivity: Hu, Rt, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PP5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PP5 Antibody - BSA Free (NBP1-87240) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: EGERTECAEPPRDEPPADGALKRAEELKTQANDYFKAKDYENAIKFYSQAIELNPSNAIYYGNRSLAYLRTECYGYALGDATRAIELDKKYIKGYYRRAASNMALGKFRAALRDYETVVKVKPHDKDAKMKYQECNKIVKQ
• Predicted Species: Mouse (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPP5C
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  PP5 Protein (NBP1-87240PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PP5 Antibody - BSA Free

• EC 3.1.3.16
• PP5
• PP5FLJ36922
• PPP5
• PPP5C
• PPT
• PP-T
• protein phosphatase 5, catalytic subunit
• Protein phosphatase T
• Serine/Threonine phosphatase
• serine/threonine-protein phosphatase 5

Background

It may play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, it dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP5C