PP4/PPP4C Antibody

Western Blot: PPP4C Antibody [NBP1-51947] - Analysis of Rat Spleen (A) and Lung (B) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: PPP4C Antibody [NBP1-51947] - (2.5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: PPP4C Antibody [NBP1-51947] - Western blot analysis of PPP4C in HEK293 lysate (35ug protein in RIPA buffer) using NBP1-51947 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Rt, Ca, Rb
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PP4/PPP4C Antibody Summary

• Immunogen: Peptide with sequence C-PQETRGIPSKKP corresponding to C-Terminus according to NP_002711.1.
• Epitope: C-PQETRGIPSKKP
• Predicted Species: Rabbit (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PPP4C
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 35 kDa band observed in HEK293, A431 and HeLa lysates and in rat spleen and lung lysates (calculated MW of 35.1 kDa band according to NP_002711.1). IHC-P: Human prostate shows nuclear staining of secretory cells in the gland.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PP4/PPP4C Antibody

• EC 3.1.3.16
• PP4
• PP4C
• PP4protein phosphatase X, catalytic subunit
• PPH3
• PPP4C
• PPX
• PP-X
• PPXPPH3
• protein phosphatase 4 (formerly X), catalytic subunit
• protein phosphatase 4, catalytic subunit
• Protein phosphatase X

Background

Protein phosphatase 4C may be involved in microtubule organization. It binds 1 iron ion and 1 manganese ion per subunit. PP4 consists of a catalytic subunit PPP4C and a regulatory subunit. PPP4R1 and belongs to the PPP phosphatase family, PP X subfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PP4/PPP4C Antibody (NBP1-51947)(1)

Publication using NBP1-51947

• Mourtada-Maarabouni M, Williams GT. Protein phosphatase 4 regulates apoptosis, proliferation and mutation rate of human cells. Biochim Biophys Acta 2008-08-01 [PMID: 18424272]

Bioinformatics

• Gene Symbol: PPP4C
• Entrez:
  • 5531(Human)
  • 171366(Rat)
• Uniprot:
  • NP_002711.1(Human)