PP2C alpha/PPM1A Antibody (7F12) - BSA Free

Western Blot: PP2C alpha/PPM1A Antibody (7F12) [NBP2-37480] - Western blot analysis using PPM1A mouse mAb against Jurkat (1), Jurkat (2), A431 (3), HeLa (4), HEK293 (5), Raji (6), MCF-7 (7), and COS7 (8) cell lysate.

Flow Cytometry: PP2C alpha/PPM1A Antibody (7F12) [NBP2-37480] - Flow cytometric analysis of HeLa cells using PPM1A mouse mAb (green) and negative control (red).

Western Blot: PP2C alpha/PPM1A Antibody (7F12) [NBP2-37480] - Western blot analysis using PPM1A mAb against human PPM1A recombinant protein. (Expected MW is 45.9 kDa)

Western Blot: PP2C alpha/PPM1A Antibody (7F12) [NBP2-37480] - Western blot analysis using PPM1A mAb against HEK293 (1) and PPM1A (AA: 202-382)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA: PP2C alpha/PPM1A Antibody (7F12) [NBP2-37480] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu, Pm, Pm
• Applications: WB, ELISA, Flow, CyTOF-ready
• Clone: 7F12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PP2C alpha/PPM1A Antibody (7F12) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human PP2C alpha/PPM1A (AA: 202-382) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPM1A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 42.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for PP2C alpha/PPM1A Antibody (7F12) - BSA Free

• EC 3.1.3.16
• FLJ42306
• MGC9201
• MMPa-2
• MPPa-1
• phosphatase 2C alpha
• PP2C alpha
• Pp2c1
• PP2CA
• PP2Calpha
• PP2C-ALPHA
• PPM1A
• PPPM1A
• protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform
• protein phosphatase 1A
• protein phosphatase 2C alpha isoform
• Protein phosphatase 2C isoform alpha
• Protein phosphatase IA
• protein phosphatase, Mg2+/Mn2+ dependent, 1A

Background

Protein phosphatase 2C (PP2C) is a family of serine/threonine specific protein phosphatases. PP2C is a 42 kDa monomeric enzyme with broad substrate specificity and its activity is depended upon divalent cations (e.g. magnesium) (1). In mammals, three members of PP2C family are known; PP2C -alpha, -beta, and -gamma. PP2C

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPM1A