POU3F2/OCT7 Antibody (761340) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB
• Clone: 761340
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

POU3F2/OCT7 Antibody (761340) [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human POU3F2
  Asn7-His59
  Accession # P20265
• Specificity: Detects human POU3F2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) POU5F1 or rhPOU4F3 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for POU3F2/OCT7 Antibody (761340) [Alexa Fluor® 532]

• Brain-2
• Brain-specific homeobox/POU domain protein 2
• BRN2
• brn-2
• BRN2brain-2
• Nervous system-specific octamer-binding transcription factor N-Oct-3
• Oct7
• oct-7
• OCT7POU domain class 3, transcription factor 2
• Octamer-binding protein 7
• Octamer-binding transcription factor 7
• OTF7
• OTF-7
• POU class 3 homeobox 2
• POU domain, class 3, transcription factor 2
• POU3F2
• POUF3

Background

POU3F2, also called Brn2, is a 47 kDa (predicted) member of the Octamer-binding transcription factor family. It is mainly expressed in the brain, especially in neuroectodermal cell lineages. With transcription factors ASCL1/Mash1 and Myt1L, POU3F2 has been used to induce neuronal differentiation of human stem cells and mouse fibroblasts. The full length transcript of POU3F2 produces the transcription factor N-Oct3, while alternate transcription initiation at amino acids 181 and 200 produce N-Oct5A and N-Oct5B isoforms, respectively. The sequence used as an immunogen is present only in N-Oct3 and is identical in human and mouse, while rat POU3F2 shows one amino acid difference.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.