POU2F3 Antibody - BSA Free

Western Blot: POU2F3 Antibody [NBP2-81774] - Western blot analysis of POU2F3 in SK-N-SH cell lysate with POU2F3 antibody at (A) 1 and (B) 2 ug/ml.

Immunocytochemistry/ Immunofluorescence: POU2F3 Antibody - BSA Free [NBP2-81774] - Immunofluorescence of POU2F3 in rat brain tissue with POU2F3 antibody at 20 ug/mL.

Immunohistochemistry: POU2F3 Antibody - BSA Free [NBP2-81774] - Immunohistochemistry of POU2F3 in rat brain tissue with POU2F3 antibody at 5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

POU2F3 Antibody - BSA Free Summary

• Immunogen: POU2F3 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human POU2F3. The immunogen is located within amino acids 370 - 420 of POU2F3. Amino Acid Squence: SPGNNSRPSSPGSGLH
• Specificity: POU2F3 antibody is human, mouse and rat reactive. At least two isoforms are known to exist. This antibody will recognize both isoforms. POU2F3 antibody is predicted to not cross-react with other members of the POU domain class 2 family.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POU2F3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin
  • Simple Western 1:40
  • Western Blot 1-2 ug/ml
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for POU2F3 Antibody - BSA Free

• Epoc-1
• MGC126698
• OCT11
• OCT-11
• OCT11FLJ40063
• Octamer-binding protein 11
• Octamer-binding transcription factor 11
• OTF11
• OTF-11
• PLA1
• PLA-1
• POU class 2 homeobox 3
• POU domain class 2, transcription factor 3
• POU domain, class 2, transcription factor 3
• POU transcription factor
• POU2F3
• Skn-1a
• Transcription factor PLA-1
• Transcription factor Skn-1

Background

POU2F3, also referred to as POU domain, class 2, transcription factor 3, is a transcription factor that belongs to the POU protein family. POU2F3 has a 436 amino acid long isoform, a 438 amino acid long isoform, and a 432 amino acid long isoform, all three of which are approximately 47kDa. POU2F3 is highly expressed in keratinocytes and the epidermis. Current research surrounding POU2F3 has shown possible interactions with diseases and disorders such as atherosclerosis, bipolar disorder, herpes, cervical cancer, breast cancer, and neuroendocrine tumors. POU2F3 has also been shown to interact with Bcl6, Ubiquitin and KAT3A/CBP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POU2F3