Porimin Antibody

Western Blot: Porimin Antibody [NB100-56371] - Analysis of Porimin using NB100-56371 at 2 ug/ml dilution against 20 ug/lane of HL60 cell lysate.

• Reactivity: Hu, Mu, Bv, Rt, Ch
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Porimin Antibody Summary

• Immunogen: This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acids 173-188 SRRGIRYRTIDEHDAI of human Porimin; GenBank no. AAG2164.1.
• Predicted Species: Rat (93%), Chicken (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TMEM123
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500. Use reported in scientific literature (PMID 16814713)
  • Western Blot 1-3 ug/ml

Reactivity Notes

Human, Mouse reactivity reported in scientific literature (PMID: 16814713).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for Porimin Antibody

• KCT3
• KCT-3
• KCT3keratinocytes associated transmembrane protein 3
• Keratinocytes-associated transmembrane protein 3
• Porimin
• PORIMINporimin
• pro oncosis receptor inducing membrane injury
• Pro-oncosis receptor inducing membrane injury
• serine/threonine-rich receptor
• TMEM123
• transmembrane protein 123PORMIN

Background

Porimin was identified by a monoclonal antibody developed by immunizing mice with apoptotic cells. One of these antibodies, designated anti-Porimin (for pro-oncosis receptor inducing membrane injury), was found to directly induce a unique type of cell death in Jurkat cells by binding to a 110-kDa cell surface receptor on Jurkat cells. Anti-Porimin-mediated cell death was preceded by cell aggregation, formation of plasma membrane pores, and the appearance of membrane blebs. More important, these cells show neither DNA fragmentation nor apoptotic bodies, but display lethal damage of the cell membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Porimin Antibody (NB100-56371)(2)

Publications using NB100-56371

• Zender L, Spector MS, Xue W et al. Identification and validation of oncogenes in liver cancer using an integrative oncogenomic approach. Cell 2006-06-30 [PMID: 16814713] (IHC-P, WB, Mouse, Human)
• Soday L Proteomic Investigation of Interferon Alpha Stimulation and Viral Regulation to Identify Candidate Antiviral Restriction Factors Thesis 2020-01-01 (FLOW, WB, Human)

Bioinformatics

• Gene Symbol: TMEM123
• Entrez:
  • 114908(Human)
  • 71929(Mouse)
  • 363013(Rat)
• Uniprot:
  • Q8N131(Human)