Porcine PDGF Protein, CF

• Reactivity: Po
• Applications: Bioactivity
• Format: Carrier-Free

Porcine PDGF Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749.

  The ED₅₀ for this effect is 6-60 ng/mL in a fluorometric assay using the redox sensitive dye, Resazurin (Catalog # AR002) and 1-3 ng/mL when measured by ³H-thymidine incorporation.

• Source: Porcine platelet-derived PDGF protein
• Protein/Peptide Type: Natural Proteins
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• SDS-PAGE: 18-28 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 50 µg/mL in sterile 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Porcine PDGF Protein, CF

• PDGF
• PDGF1
• PDGF-1
• PDGFA
• PDGF-A

Background

PDGF was originally discovered as a major mitogenic factor in serum but not in plasma. PDGF is stored in platelet alpha  granules and released upon platelet activation. Besides megakaryocytes, other cell types, including endothelial cells, monocyte/macrophages, vascular smooth muscle cells, fibroblasts, cytotrophoblasts and a variety of transformed or neoplastic cells, have been shown to produce PDGF. PDGFs are disulfide-linked dimers. The subunits of the PDGF dimers are homologous polypeptides designated PDGF-A and PDGF-B chains. Natural PDGFs can exist either as homodimers (PDGF-AA, PDGF-BB) or heterodimers (PDGF-AB). Although all three isoforms of PDGF exist in human platelets, R&D Systems’ hPDGF consists predominantly of hPDGF-AB heterodimers. PDGF obtained from porcine platelets contain only PDGF-BB homodimers.

Two distinct PDGF receptors, the alpha -receptor and the beta -receptor, have been identified. The two receptors are structurally related, with an extracellular portion containing five immunoglobulin-like domains, a single transmembrane region, and an intracellular portion with a protein-tyrosine kinase domain. The alpha -receptor binds both the A and B chains with high affinity whereas the beta -receptor binds only the B-chain with high affinity. Receptor dimerization is induced upon ligand binding.

In addition to being a potent mitogen for cells of mesenchymal origin, PDGF has also been shown to be a potent chemoattractant for mesenchymal cells, mononuclear cells and neutrophils and has been reported to be important in the modification of cellular matrix constituents.

Publications for PDGF (125-PD)(3)

Publications using 125-PD

• T Sakabe, K Sakai, T Maeda, A Sunaga, N Furuta, R Schweitzer, T Sasaki, T Sakai Transcription factor scleraxis vitally contributes to progenitor lineage direction in wound healing of adult tendon in mice J. Biol. Chem., 2018-03-05;0(0):. 2018-03-05 [PMID: 29507095] (Bioassay, Mouse)
• Ye L, Zhang P, Duval S, Su L, Xiong Q, Zhang J Thymosin beta4 increases the potency of transplanted mesenchymal stem cells for myocardial repair. Circulation, 2013-09-10;128(11):S32-41. 2013-09-10 [PMID: 24030419] (Bioassay, Porcine)
• Tsoporis JN, Izhar S, Parker TG Expression of S100A6 in cardiac myocytes limits apoptosis induced by tumor necrosis factor-alpha. J. Biol. Chem., 2008-08-27;283(44):30174-83. 2008-08-27 [PMID: 18753141] (Bioassay, Rat)