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POR/Cytochrome P450 Reductase Antibody

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Western Blot: POR/Cytochrome P450 Reductase Antibody [H00005447-B01P] - Analysis of POR expression in human liver.
Western Blot: POR/Cytochrome P450 Reductase Antibody [H00005447-B01P] - Analysis of POR expression in human liver.
Immunocytochemistry/ Immunofluorescence: POR/Cytochrome P450 Reductase Antibody [H00005447-B01P] - Analysis of purified antibody to POR on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: POR/Cytochrome P450 Reductase Antibody [H00005447-B01P] - Analysis of POR expression in transfected 293T cell line by POR polyclonal antibody. Lane 1: POR transfected lysate(74.8 KDa). Lane 2: ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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POR/Cytochrome P450 Reductase Antibody Summary

Immunogen
POR (AAH34277.1, 1 a.a. - 680 a.a.) full-length human protein. MINMGDSHVDTSSTVSEAVAEEVSLFSMTDMILFSLIVGLLTYWFLFRKKKEEVPEFTKIQTLTSSVRESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEEYDLADLSSLPEIDNALVVFCMATYGEGDPTDNAQDFYDWLQETDVDLSGVKFAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFELGLGDDDGNLEEDFITWREQFWLAVCEHFGVEATGEESSIRQYELVVHTDIDAAKVYMGEMGRLKSYENQKPPFDAKNPFLAAVTTNRKLNQGTERHLMHLELDISDSKIRYESGDHVAVYPANDSALVNQLGKILGADLDVVMSLNNLDEESNKKHPFPCPTSYRTALTYYLDITNPPRTNVLYELAQYASEPSEQELLRKMASSSGEGKELYLSWVVEARRHILAILQDCPSLRPPIDHLCELLPRLQARYYSIASSSKVHPNSVHICAVVVEYETKAGRINKGVATNWLRAKEPVGENGGRALVPMFVRKSQFRLPFKATTPVIMVGPGTGVAPFIGFIQERAWLRQQGKEVGETLLYYGCRRSDEDYLYREELAQFHRDGALTQLNVAFSREQSHKVYVQHLLKQDREHLWKLIEGGAHIYVCGDARNMARDVQNTFYDIVAELGAMEHAQAVDYIKKLMTKGRYSLDVWS
Specificity
POR - P450 (cytochrome) oxidoreductase,
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
POR
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It is also useful for IF.
Control
POR/Cytochrome P450 Reductase Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for POR/Cytochrome P450 Reductase Antibody

  • CPR
  • CYPOR
  • CYPORFLJ26468
  • Cytochrome P450 Reductase
  • DKFZp686G04235
  • EC 1.6.2.4
  • NADPH--cytochrome P450 reductase
  • NADPH-dependent cytochrome P450 reductase
  • P450 (cytochrome) oxidoreductase
  • P450R
  • POR

Background

This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
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44110002
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POR/Cytochrome
P450 Reductase Antibo
H00005447-B01P
POR/Cytochrome P450 Reductase Antibody
Species: Hu
Applications: WB, ICC/IF

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Control Lysate(s)

POR/Cytochrome P450 Reductase Knockout HeLa Cell Lysate

Secondary Antibodies

 

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Bioinformatics

Gene Symbol POR
Entrez
Uniprot