POR/Cytochrome P450 Reductase Antibody (JB54-31)

Western Blot: POR/Cytochrome P450 Reductase Antibody (JB54-31) [NBP2-75626] - Analysis of Cytochrome P450 Reductase on SK-Br-3 (1) , A549 (2) and mouse lung (3) lysate using anti-Cytochrome P450 Reductase antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: POR/Cytochrome P450 Reductase Antibody (JB54-31) [NBP2-75626] - Staining Cytochrome P450 Reductase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody (JB54-31) [NBP2-75626] - Analysis of paraffin-embedded rat brain tissue using anti-Cytochrome P450 Reductase antibody. Counter stained with hematoxylin.

Flow Cytometry: POR/Cytochrome P450 Reductase Antibody (JB54-31) [NBP2-75626] - Analysis of HepG2 cells with Cytochrome P450 Reductase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: POR/Cytochrome P450 Reductase Antibody (JB54-31) [NBP2-75626] - Staining Cytochrome P450 Reductase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: POR/Cytochrome P450 Reductase Antibody (JB54-31) [NBP2-75626] - Staining Cytochrome P450 Reductase in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody (JB54-31) [NBP2-75626] - Analysis of paraffin-embedded human liver tissue using anti-Cytochrome P450 Reductase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody (JB54-31) [NBP2-75626] - Analysis of paraffin-embedded human lung cancer tissue using anti-Cytochrome P450 Reductase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody (JB54-31) [NBP2-75626] - Analysis of paraffin-embedded mouse testis tissue using anti-Cytochrome P450 Reductase antibody. Counter stained with hematoxylin.

Product Discontinued

POR/Cytochrome P450 Reductase Antibody (JB54-31) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human POR/Cytochrome P450 Reductase aa 80-220 / 677. (SwissProt: P16435 Human; SwissProt: P37040 Mouse; SwissProt: P00388 Rat)
• Localization: Endoplasmic reticulum.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: POR
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 77 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for POR/Cytochrome P450 Reductase Antibody (JB54-31)

• CPR
• CYPOR
• CYPORFLJ26468
• Cytochrome P450 Reductase
• DKFZp686G04235
• EC 1.6.2.4
• NADPH--cytochrome P450 reductase
• NADPH-dependent cytochrome P450 reductase
• P450 (cytochrome) oxidoreductase
• P450R
• POR

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POR