POR/Cytochrome P450 Reductase Antibody (739613) [Alexa Fluor® 488]

• Applications: WB
• Clone: 739613
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

POR/Cytochrome P450 Reductase Antibody (739613) [Alexa Fluor® 488] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human POR/Cytochrome P450 Reductase
  Arg45-Ser677
  Accession # P16435
• Specificity: Detects human POR/Cytochrome P450 Reductase in direct ELISAs.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

NADPH-Cytochrome P450 Reductase (POR, P450R) is an essential component of the cytochrome P450 monooxygenase system of eukaryotic cells (1). POR is anchored in the endoplasmic reticulum membrane with its catalytic domain residing in the cytosol. POR is a flavoprotein, containing one molecule each of FMN and FAD, which are essential for the transfer of electrons from NADPH to the cytochromes P450 (2). This reduction is necessary for cytochromes P450 to perform each cycle of oxidation. POR is also capable of transferring electrons to cytochrome b₅, heme oxygenase, the fatty acid elongation system, and other proteins. Mutations of POR can result in disordered steroidogenesis and Antley-Bixler syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.