POP1 Antibody

Images

 
Immunocytochemistry: POP1 Antibody [NBP1-26606] - FFPE section of human testicular seminoma. Antibody used at a dilution of 1:100. Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed ...read more
Immunohistochemistry: POP1 Antibody [NBP1-26606] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- POP1 used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: POP1 Antibody [NBP1-26606] - FFPE section of human prostate carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunohistochemistry-Paraffin: POP1 Antibody [NBP1-26606] - FFPE section of human skin tumor. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunoprecipitation: POP1 Antibody [NBP1-26606] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit used for IP at 10 mcg/mg lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC, IHC-P, IP, ICC/IF, WB (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

POP1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 925 and 975 of human processing of precursor 1 using the numbering given in entry NP_055844.1 (GeneID 10940).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:2000 - 1:10000
  • Immunoprecipitation 5-10 ug/mg lysate
  • Immunocytochemistry
Application Notes
It is not recommended for western analysis. Immunohistochemistry 1:2,000 to 1:10,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for POP1 Antibody

  • EC 3.1.26.5
  • hPOP1
  • KIAA0061
  • MGC17365
  • processing of precursor 1, ribonuclease P/MRP subunit (S. cerevisiae)
  • processing of precursors 1
  • ribonucleases P/MRP protein subunit POP1

Background

POP1 (processing of precursor 1) is a subunit of both the ribonuclease P and RNase MRP holoenzymes which are involved in 5'-end maturation of precursor tRNAs (pre-tRNAs). POP1 has been found to be an autoantigen in patients suffering from connective tissue diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol POP1
Entrez
Uniprot