POLR2K Overexpression Lysate

Images

 
Western Blot: POLR2K Overexpression Lysate (Adult Normal) [NBL1-14587] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for POLR2K.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

POLR2K Overexpression Lysate Summary

Description

POLR2K Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC201957

Accession#: NM_005034

Protein Tag: C-MYC/DDK
Gene
POLR2K

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
6.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for POLR2K Overexpression Lysate

  • ABC10-alpha
  • DNA directed RNA polymerases I, II, and III 7.0 kda polypeptide
  • DNA-directed RNA polymerase II subunit K
  • DNA-directed RNA polymerases I, II, and III subunit RPABC4
  • hRPB7.0
  • hsRPB10a
  • polymerase (RNA) II (DNA directed) polypeptide K, 7.0kDa
  • RNA polymerase II 7.0 kDa subunit
  • RNA polymerases I, II, and III subunit ABC4
  • RPABC4
  • RPB10alphapolymerase (RNA) II (DNA directed) polypeptide K (7.0kD)
  • RPB12
  • RPB7.0

Background

This gene encodes one of the smallest subunits of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for POLR2K Lysate (NBL1-14587) (0)

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Bioinformatics

Gene Symbol POLR2K