POLR2F Overexpression Lysate (Native)

Images

 
Western Blot: POLR2F Overexpression Lysate (Adult Normal) [NBL1-14583] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for POLR2F.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

POLR2F Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200855 and based on accession number NM_021974. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens polymerase (RNA) II (DNA directed) polypeptide F (POLR2F), mRNA.
Gene
POLR2F

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
14.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for POLR2F

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for POLR2F Overexpression Lysate (Native)

  • DNA-directed RNA polymerase II subunit F
  • DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide
  • HRBP14.4
  • II, and III subunit RPABC2
  • polymerase (RNA) II (DNA directed) polypeptide F
  • RNA Polymerase II subunit 14.4 kD
  • RNA polymerases I, II, and III subunit ABC2
  • RPB6 homolog
  • RPB6

Background

This gene encodes the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes, that is also shared by the other two DNA-directed RNA polymerases. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. [provided by RefSeq]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no quality transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for POLR2F Lysate (NBL1-14583) (0)

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Bioinformatics

Gene Symbol POLR2F