Recombinant Human POLR2E His Protein

SDS-Page: POLR2E Protein [NBP2-23344]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human POLR2E His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-210 of Human POLR2E
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMDDEEET YRLWKIRKTI MQLCHDRGYL VTQDELDQTL EEFKAQFGDK PSEGRPRRTD LTVLVAHNDD PTDQMFVFFP EEPKVGIKTI KVYCQRMQEE NITRALIVVQ QGMTPSAKQS LVDMAPKYIL EQFLQQELLI NITEHELVPE HVVMTKEEVT ELLARYKLRE NQLPRIQAGD PVARYFGIKR GQVVKIIRPS ETAGRYITYR LVQ

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: POLR2E
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human POLR2E His Protein

• DNA directed RNA polymerase II 23 kda polypeptide
• DNA-directed RNA polymerase II 23 kDa polypeptide
• DNA-directed RNA polymerase II subunit E
• DNA-directed RNA polymerases I, II, and III subunit RPABC1
• hRPB25
• hsRPB5
• polymerase (RNA) II (DNA directed) polypeptide E (25kD)
• polymerase (RNA) II (DNA directed) polypeptide E, 25kDa
• RPABC1
• RPB5 homolog
• RPB5
• XAP4RNA polymerases I, II, and III subunit ABC1

Background

POLR2E, also as known as DNA-directed RNA polymerases I, II, and III subunit RPABC1, belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family. POLR2E is the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. POLR2E is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Recombinant human POLR2E protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: POLR2E