Novus Biologicals products are now on bio-techne.com

POLR1D Overexpression Lysate

Images

 
Western Blot: POLR1D Overexpression Lysate (Adult Normal) [NBL1-14577] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for POLR1D.
Western Blot: POLR1D Overexpression Lysate (Adult Normal) [NBL1-14577] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for POLR1D.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

POLR1D Overexpression Lysate Summary

Description

POLR1D Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC201466

Accession#: NM_015972

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
POLR1D

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
15.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for POLR1D Overexpression Lysate

  • AC19
  • DNA-directed RNA polymerase I subunit D
  • DNA-directed RNA polymerases I and III subunit RPAC2
  • FLJ20616
  • hRPA19
  • MGC9850
  • POLR1C
  • polymerase (RNA) I polypeptide D, 16kDa
  • RPA16RNA polymerase I 16 kDa subunit
  • RPA9
  • RPAC2RNA polymerases I and III subunit AC2
  • RPC16
  • RPO1-3
  • TCS2

Background

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common core component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-80817
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-93617
Species: Hu, Mu
Applications: ICC/IF, WB
NBP1-86908
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-53092
Species: Hu, Mu
Applications: ChIP, ICC/IF, IHC, IHC-P, WB
NBP1-84628
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-89314
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP2-93863
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
H00080279-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
NBP1-83204
Species: Hu
Applications: ICC/IF, WB
NBP1-87113
Species: Dr, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB600-302
Species: Bv, Dr, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, S-ELISA, Simple Western, WB
NB300-268
Species: Bv, Ce, Hu, I, Mu, Pl
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO, WB
NBP1-30032
Species: Bv, Ca, Fe, Hu, Mu, Xp
Applications: ICC/IF, IHC, IHC-Fr, KO, WB
NB600-607
Species: Hu, Rt
Applications: ELISA, IHC, IHC-P, WB
NBP1-31649
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-00482
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-32716
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
NBP1-80816
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NB200-598
Species: Hu, Mu, Pm(-), Ye
Applications: ChIP, CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IP, WB
NBP1-91642
Species: Hu
Applications: ICC/IF, IHC, IHC-P

Publications for POLR1D Lysate (NBL1-14577) (0)

There are no publications for POLR1D Lysate (NBL1-14577).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for POLR1D Lysate (NBL1-14577) (0)

There are no reviews for POLR1D Lysate (NBL1-14577). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for POLR1D Lysate (NBL1-14577) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional POLR1D Products

Blogs on POLR1D

There are no specific blogs for POLR1D, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our POLR1D Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol POLR1D