![Immunoprecipitation: POC5 Antibody [NBP1-78740] - Whole cell lysate (1 mg for IP, 20 percent of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-POC5 antibody NBP1-78740 used for IP at 6 ug/mg lysate. POC5 was also immunoprecipitated by rabbit anti-POC5 antibody NBP1-78741, which recognizes a downstream epitope. For blotting immunoprecipitated POC5, NBP1-78740 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/POC5-Antibody-Immunoprecipitation-NBP1-78740-img0004.jpg "Immunoprecipitation: POC5 Antibody [NBP1-78740] - Whole cell lysate (1 mg for IP, 20 percent of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-POC5 antibody NBP1-78740 used for IP at 6 ug/mg lysate. POC5 was also immunoprecipitated by rabbit anti-POC5 antibody NBP1-78741, which recognizes a downstream epitope. For blotting immunoprecipitated POC5, NBP1-78740 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
| Reactivity | HuSpecies Glossary |
| Applications | IP, WB (-) |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Rabbit POC5 Antibody - BSA Free (NBP1-78740) is a polyclonal antibody validated for use in IP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The immunogen for this product maps to a region between residue 1 and 50 of human Protein of Centriole 5 using the numbering given in entry NP_001092741.1 (GeneID 134359). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | POC5 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody has not performed satisfactrily when used for WB of POC5 in crude preparations (e.g. whole cell lysate), but can be used for WB of enriched (e.g. immunoprecipitated) sources of POC5. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
![Western Blot PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Western_Blot_11082.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11144.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11480.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | POC5 |
| Uniprot |
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![N/A BDNF [HRP]](https://images.novusbio.com/images2/DATA_BDNF_DBD00_ELISA_587.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/DATA_BDNF_DBD00_ELISA_588.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/BDNF_DBD00_ELISA_36.jpg)
![Immunohistochemistry Neurexin 3/NRXN3 Antibody [Unconjugated]](https://images.novusbio.com/images2/af5269_human-mouse-neurexin-3-affinity-purified-polyclonal-ab-immunohistochemistry-2682022134435.jpg)
![Western Blot Neurexin 3/NRXN3 Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af5269_human-mouse-neurexin-3-affinity-purified-polyclonal-ab-western-blot-612023112046.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")