PNUTS/PPP1R10 Antibody - BSA Free

Western Blot: PNUTS/PPP1R10 Antibody [NB100-605] - Detection of Human PNUTS by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PNUTS antibody NB100-605 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: PNUTS/PPP1R10 Antibody [NB100-605] - Detection of Human PNUTS on HeLa whole cell lysates usings NB100-605. IP of PNUTS was successfully performed with NB100-603, NB100-604, and another PNUTS polyclonal. IPed PNUTS was blotted using NB100-605

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PNUTS/PPP1R10 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PNUTS/PPP1R10 Antibody - BSA Free (NB100-605) is a polyclonal antibody validated for use in WB and IP. Anti-PNUTS/PPP1R10 Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide, mapping to a region region between residue 900 and the C-terminus (residue 940) of human Phosphatase Nuclear Targeting Subunit (a.k.a. Protein Phosphatase 1, regulatory subunit 10) using the numbering given in TrEMBL entry Q96QC0 (GeneID 5514).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPP1R10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-10 ug/mg lysate
  • Western Blot 1:1000-1:5000
• Application Notes: NB 100-605 may be used for Western blot. Suggested working dilutions: * Western Blot: 1:1,000 - 1:5,000 Immunoprecipitation: 1 to 10 ug/mg lysate Histo/Cytochemistry: not tested. *The investigator should determine the optimal working dilution for a specific application.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:31877321).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PNUTS/PPP1R10 Antibody - BSA Free

• CAT53
• FB19PP1-binding protein of 114 kDa
• MHC class I region proline-rich protein CAT53
• p99
• Phosphatase 1 nuclear targeting subunit
• phosphatase nuclear targeting subunit
• PNUTS
• PNUTSPP1R10
• PPP1R10
• Protein FB19
• protein phosphatase 1 regulatory subunit 10
• protein phosphatase 1, regulatory (inhibitor) subunit 10
• protein phosphatase 1, regulatory subunit 10
• serine/threonine-protein phosphatase 1 regulatory subunit 10

Background

Protein phosphatase 1 (PP1) is found in the cell nucleus and has been implicated in several aspects of nuclear function. Phosphatase 1 nuclear targeting subunit (PNUTS) is a novel protein that exhibits a discreet nuclear compartmentalization and is colocalized with chromatin at distinct phases during mitosis. The subcellular localization of PP1 and the activity toward substrates involved in many aspects of cell physiology have previously been shown to be regulated by association with noncatalytic targeting subunits. The properties of PNUTS are consistent with its role as a targeting subunit for the regulation of nuclear PP1 function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PNUTS/PPP1R10 Antibody (NB100-605)(6)

Publications using NB100-605

• Kwon J, Yeh Y, Kawarasaki S et al. Mevalonate biosynthesis pathway regulates the development and survival of brown adipocytes iScience 2023-03-01 [PMID: 36895651]
• Jing X, Wu J, Dong C et al. COVID-19 instigates adipose browning and atrophy through VEGF in small mammals Nature metabolism 2022-12-01 [PMID: 36482111]
• Panagiotakopoulou V, Botsakis K, Delis F et al. Anti-neuroinflammatory, protective effects of the synthetic microneurotrophin BNN-20 in the advanced dopaminergic neurodegeneration of "weaver" mice Neuropharmacology 2020-03-15 [PMID: 31877321] (WB, Mouse)
• Pathak, P;Chiang, JYL; Sterol 12 alpha-hydroxylase Aggravates Dyslipidemia by Activating the Ceramide/mTORC1/SREBP1C Pathway via FGF21 and FGF15 Gene Expr. 2019-03-19 [PMID: 30890204]
• F Nakazeki, M Nishiga, T Horie, H Nishi, Y Nakashima, O Baba, Y Kuwabara, T Nishino, T Nakao, Y Ide, S Koyama, M Kimura, S Tsuji, N Sowa, S Yoshida, SJ Conway, M Yanagita, T Kimura, K Ono Loss of periostin ameliorates adipose tissue inflammation and fibrosis in vivo Sci Rep, 2018-06-04;8(1):8553. 2018-06-04 [PMID: 29867212]
• Travis B McMurphy Hepatic Expression of Adenovirus 36 E4ORF1 Improves Glycemic Control and Promotes Glucose Metabolism via AKT Activation Diabetes, 2016-11-30;0(0):. 2016-11-30 [PMID: 27903748]

Bioinformatics

• Gene Symbol: PPP1R10
• Entrez:
  • 5514(Human)
• Uniprot:
  • Q96QC0(Human)