PNUTS/PPP1R10 Antibody [Unconjugated]

Western blot shows lysates of TS1 mouse helper T cell line and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat PNUTS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF21581) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PNUTS at approximately 114 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for PNUTS at approximately 100-150 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human/Mouse/Rat PNUTS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF21581). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

PNUTS/PPP1R10 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PNUTS
  Pro5-Asn226
  Accession # NP_002705
• Specificity: Detects human, mouse, and rat PNUTS in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPP1R10
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 1 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PNUTS/PPP1R10 Antibody [Unconjugated]

• CAT53
• FB19PP1-binding protein of 114 kDa
• MHC class I region proline-rich protein CAT53
• p99
• Phosphatase 1 nuclear targeting subunit
• phosphatase nuclear targeting subunit
• PNUTS
• PNUTSPP1R10
• PPP1R10
• Protein FB19
• protein phosphatase 1 regulatory subunit 10
• protein phosphatase 1, regulatory (inhibitor) subunit 10
• protein phosphatase 1, regulatory subunit 10
• serine/threonine-protein phosphatase 1 regulatory subunit 10

Background

PP1 nuclear targeting subunit (PNUTS), also known as PPP1R10 and FB19, is a 99 kDa protein that binds the serine/threonine protein phosphatase PP1 and causes its localization to chromatin in the nucleus. C-terminal RGG repeats in PNUTS suggest it may bind to single stranded RNA. The PP1/PNUTS complex may also play a role in regulating the cell cycle-dependent phosphorylation of the oncogene Rb.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP1R10
• Uniprot:
  • NP_002705()