PNMT Antibody (1C11) - BSA Free

Western Blot: PNMT Antibody (1C11) [NBP1-97942] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PNMT antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunocytochemistry/Immunofluorescence: PNMT Antibody (1C11) [NBP1-97942] - Analysis in Hep3B cells. The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western Blot: PNMT Antibody (1C11) [NBP1-97942] - Lane 1.: 293T cell lysate Lane 2.: PNMT transfected 293T cell lysate

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 1C11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PNMT Antibody (1C11) - BSA Free Summary

• Description: Novus Biologicals Mouse PNMT Antibody (1C11) - BSA Free (NBP1-97942) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human PNMT (1-282aa) purified from E. coli
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PNMT
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for PNMT Antibody (1C11) - BSA Free

• EC 2.1.1
• EC 2.1.1.28
• Noradrenaline N-methyltransferase
• PENT
• PENTMGC34570
• phenylethanolamine N-methylase
• phenylethanolamine N-methyltransferase
• PNMT
• PNMTase

Background

Phenylethanolamine N-methyltransferase (PNMT) catalyzes the synthesis of epinephrine and plays an important role in regulating cardiovascular function. Expression of PNMT is regulated by hormonal and neural stimuli because its promoter contains sequences responding to cholinergic and depolarization stimuli. PNMT is the final enzyme in the catecholamine pathway, catalyzing the conversion of norepinephrine to epinephrine using as the cosubstrate and methyl donor, S-adenosylmethionine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PNMT
• Uniprot:
  • NP_002677(Human)