PNK Antibody

Western Blot: PNK Antibody [NBP1-32764] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE, antibody diluted at 1:2000.

Immunohistochemistry-Paraffin: PNK Antibody [NBP1-32764] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.

Western Blot: PNK Antibody [NBP1-32764] - Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000

Product Discontinued

PNK Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human PNK. The exact sequence is proprietary.
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PNKP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for PNK Antibody

• DNA 5'-kinase/3'-phosphatase
• EIEE10
• Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP)
• MCSZ
• PNK
• PNKbifunctional polynucleotide phosphatase/kinase
• PNKP
• polynucleotide kinase 3'-phosphatase
• Polynucleotide kinase-3'-phosphatase

Background

Mammalian PNK (also known as PNKP, Polynucleotide kinase 3'-phosphatase, Polynucleotide kinase 3-prime-phosphatase, DNA 5'-kinase/3'-phosphatase and Polynucleotide Kinase) catalyzes the 5- prime phosphorylation of nucleic acids and can have associated 3-prime phosphatase activity, predictive of an important function in DNA repair following ionizing radiation or oxidative damage. PNK is a nuclear protein that is expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PNKP
• Entrez:
  • 11284(Human)
• Uniprot:
  • Q96T60(Human)