PNK Antibody

Immunohistochemistry-Paraffin: PNK Antibody [NBP1-02450] - Staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Product Discontinued

PNK Antibody Summary

• Immunogen: Synthetic peptide from human PNK.
• Predicted Species: Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PNKP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:3000-1:12000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1:300-1:1500

Reactivity Notes

Predicted reactivity based on percent identity from BLAST analysis: Canine (83%), Mouse (83%), Rat (83%)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
• Preservative: 0.01% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PNK Antibody

• DNA 5'-kinase/3'-phosphatase
• EIEE10
• Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP)
• MCSZ
• PNK
• PNKbifunctional polynucleotide phosphatase/kinase
• PNKP
• polynucleotide kinase 3'-phosphatase
• Polynucleotide kinase-3'-phosphatase

Background

Mammalian PNK (also known as PNKP, Polynucleotide kinase 3'-phosphatase, DNA 5'-kinase/3'-phosphatase and Polynucleotide kinase) catalyzes the 5 phosphorylation of nucleic acids and can have associated 3 phosphatase activity, predictive of an important function in DNA repair following ionizing radiation or oxidative damage. PNK is a nuclear protein that is expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PNKP
• Entrez:
  • 59047(Mouse)
  • 308576(Rat)
• Uniprot:
  • Q96T60(Human)