PNK Antibody

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Immunohistochemistry-Paraffin: PNK Antibody [NBP1-02450] - Staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

PNK Antibody Summary

Immunogen
Synthetic peptide from human PNKP.
Specificity
An internal region of human PNK protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PNKP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:300-1:1500
  • ELISA 1:3000-1:12000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PNK Antibody

  • DNA 5'-kinase/3'-phosphatase
  • EIEE10
  • Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP)
  • MCSZ
  • PNKbifunctional polynucleotide phosphatase/kinase
  • polynucleotide kinase 3'-phosphatase
  • Polynucleotide kinase-3'-phosphatase

Background

Mammalian PNK (also known as PNKP, Polynucleotide kinase 3'-phosphatase, DNA 5'-kinase/3'-phosphatase and Polynucleotide kinase) catalyzes the 5 phosphorylation of nucleic acids and can have associated 3 phosphatase activity, predictive of an important function in DNA repair following ionizing radiation or oxidative damage. PNK is a nuclear protein that is expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PNKP
Entrez
Uniprot