PNK Antibody

Product Discontinued

PNK Antibody Summary

• Immunogen: Peptide with sequence EPRLGRLYCQFSEG corresponding to C-Terminus according to NP_009185.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PNKP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:200
  • Peptide ELISA Detection limit 1:16000
• Application Notes: WB: Sucessfully used to detect recombinant human PNKP (1/1000 dilution).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PNK Antibody

• DNA 5'-kinase/3'-phosphatase
• EIEE10
• Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP)
• MCSZ
• PNK
• PNKbifunctional polynucleotide phosphatase/kinase
• PNKP
• polynucleotide kinase 3'-phosphatase
• Polynucleotide kinase-3'-phosphatase

Background

Mammalian PNK catalyzes the 5-prime phosphorylation of nucleic acids and can have associated 3-prime phosphatase activity, predictive of an important function in DNA repair following ionizing radiation or oxidative damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PNK Antibody (NB100-1067)(1)

Publication using NB100-1067

• Jilani A, Ramotar D, Slack C et al. Molecular cloning of the human gene, PNKP, encoding a polynucleotide kinase 3'-phosphatase and evidence for its role in repair of DNA strand breaks caused by oxidative damage. J Biol Chem 1999-08-20 [PMID: 10446192]

Bioinformatics

• Gene Symbol: PNKP
• Entrez:
  • 11284(Human)
• Uniprot:
  • NP_009185.(Human)