PMCA1 Antibody - BSA Free

Western Blot: PMCA1 Antibody [NBP3-12964] - PMCA1 antibody detects PMCA1 protein by western blot analysis. HeLa whole cell extracts and membrane extracts(the extracts were unboiled )(30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PMCA1 antibody (NBP3-12964) at a dilution of 1:1000.

Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP3-12964] - PMCA1 antibody detects PMCA1 protein expression by immunohistochemical analysis. Sample:Paraffin-Embedded adult mouse retina. Green: PMCA1 protein stained by PMCA1 antibody (NBP3-12964) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP3-12964] - PMCA1 antibody detects PMCA1 protein at cell membrane and cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. PMCA1 antibody (NBP3-12964) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP3-12964] - PMCA1 antibody detects PMCA1 protein at cell membrane and cytoplasm in rat prostate by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. PMCA1 antibody (NBP3-12964) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Boiled and unboiled A549 whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PMCA1 antibody (NBP3-12964) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Rb
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PMCA1 Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PMCA1. The exact sequence is proprietary.
• Predicted Species: Porcine (100%), Bovine (98%), Rabbit (98%), Canine (100%), Chicken (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP2B1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Application Notes: IHC-FR-Assay dependent
• Theoretical MW: 139 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for PMCA1 Antibody - BSA Free

• ATPase, Ca++ transporting, plasma membrane 1
• EC 3.6.3
• EC 3.6.3.8
• Plasma membrane calcium ATPase isoform 1
• plasma membrane calcium ATPase
• Plasma membrane calcium pump isoform 1
• plasma membrane calcium pump
• plasma membrane calcium-ATPase
• PMCA1kb
• PMCA1plasma membrane calcium-transporting ATPase 1

Background

The calcium pump of the plasma membrane (PMCA ATPase) pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the sarcoplasmic and endoplasmic reticulum calcium (SERCA) ATPase and the sodium/potassium pump. Studies indicate that there are four known isoforms of the PMCA ATPase which are encoded by a multigene family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP2B1