PLUNC Antibody (251512)

• Reactivity: Hu
• Applications: WB
• Clone: 251512
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PLUNC Antibody (251512) Summary

• Immunogen: E. coli-derived recombinant human PLUNC
  Gln20-Val256
  Accession # Q9NP55
• Specificity: Detects human PLUNC in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: BPIFA1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PLUNC Antibody (251512)

• bA49G10.5
• BPIFA1
• ligand-binding protein RYA3
• LPLUNC3
• Lung-specific protein X
• LUNX
• LUNXNASG
• Nasopharyngeal carcinoma-related protein
• Palate lung and nasal epithelium clone protein
• palate, lung and nasal epithelium associated
• palate, lung and nasal epithelium carcinoma associated
• PLUNC
• protein Plunc
• Secretory protein in upper respiratory tracts
• SPLUNC1
• SPLUNC1SPURT
• tracheal epithelium enriched protein
• Tracheal epithelium-enriched protein
• Von Ebner protein Hl

Background

PLUNC, also named SPLUNC1, is a secreted protein that is expressed in the secretory ducts and submucosal glands of tracheobronchial tissues. It is structurally related to bactericidal/permeability-increasing protein (BPI) and lipopolysaccaride binding protein (LBP), which are central to the host defense against gram-negative bacteria. PLUNC belongs to the short subfamily of PLUNC family proteins and has homology only to the N-terminal domains of BPI. PLUNC may function in the innate immune response against bacteria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PLUNC Antibody (MAB1897)(7)

Publications using MAB1897

• Yung-An Tsou, Chia-Der Lin, Hui-Chen Chen, Hui-Ying Hsu, Lii-Tzu Wu, Chuan Chiang-Ni et al. Interleukin-13 Inhibits Lipopolysaccharide-Induced BPIFA1 Expression in Nasal Epithelial Cells PLOS ONE 12/8/2015 [PMID: 26646664]
• Jennifer A. Bartlett, David K. Meyerholz, Christine L. Wohlford-Lenane, Paul W. Naumann, Nita H. Salzman, Paul B. McCray Increased susceptibility to otitis media in a Splunc1-deficient mouse model Disease Models & Mechanisms 5/1/2015 [PMID: 25765466]
• Liu Y, Bartlett J, Di M, Bomberger J, Chan Y, Gakhar L, Mallampalli R, McCray P, Di Y SPLUNC1/BPIFA1 contributes to pulmonary host defense against Klebsiella pneumoniae respiratory infection. Am J Pathol, 2013-03-15;182(5):1519-31. 2013-03-15 [PMID: 23499554] (Western Blot, Human)
• Pezzulo AA, Tang XX, Hoegger MJ, Alaiwa MH, Ramachandran S, Moninger TO, Karp PH, Wohlford-Lenane CL, Haagsman HP, van Eijk M, Banfi B, Horswill AR, Stoltz DA, McCray PB, Welsh MJ, Zabner J Reduced airway surface pH impairs bacterial killing in the porcine cystic fibrosis lung. Nature, 2012-07-04;487(7405):109-13. 2012-07-04 [PMID: 22763554] (Western Blot, Porcine)
• Karl G. Kohlgraf, Abbey R. Ackermann, Kindra K. Burnell, Rupasree N. Srikantha, Sophie A. Joly, Jennifer A. Bartlett et al. Quantitation of SPLUNC1 in saliva with an xMAP particle-based antibody capture and detection immunoassay Archives of Oral Biology 2/1/2012 [PMID: 21925642]
• McGillivary G, Bakaletz LO The multifunctional host defense peptide SPLUNC1 is critical for homeostasis of the mammalian upper airway. PLoS ONE, 2010-10-07;5(10):e13224. 2010-10-07 [PMID: 20949060] (Western Blot, Chinchilla)
• Steiling K, Kadar AY, Bergerat A Comparison of proteomic and transcriptomic profiles in the bronchial airway epithelium of current and never smokers. PLoS ONE, 2009-04-09;4(4):e5043. 2009-04-09 [PMID: 19357784] (Western Blot, Human)

Bioinformatics

• Gene Symbol: BPIFA1
• Uniprot:
  • Q9NP55()