PLUNC Antibody (251512)

JNK/c-Jun pathway is involved in LPS-mediated up-regulation of BPIFA1 expression in nasal epithelial cells.(A) Cells were pretreated for 1 h with 20 μM PD98059 (ERK inhibitor), 10 μM SP600125 (JNK inhibitor), or 20 μM SB203580 (p38 inhibitor) and incubated with 10 μg/ml LPS for 2 h. (B) Cells were transfected with a JNK-dominant negative (DN-JNK) mutant for 24 h or pretreated with SP600125 for 30 min prior to incubation with LPS for 1 h. The protein expression levels were determined using western blot. beta -actin was used as the loading control. The western blots were carried out independently in triplicate and results were representative of one of three independent experiments. The expression level of each protein was quantified by signal intensity and was indicated at the bottom of each lane. The quantitative analysis of western blot for three independent experiments was shown in S2 Fig. ANOVA with Tukey’s test was used to compare the overall difference between the groups. P < 0.05 was considered statistically significant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26646664), licensed under a CC-BY license. Not internally tested by R&D Systems.

AP-1 is involved in LPS-induced BPIFA1 expression. Cells were pretreated with 10 μM curcumin or tanshinone (inhibitors of c-Jun) for 30 min, followed by incubation with LPS (10 μg/ml) for 2 h. Protein expression levels were determined using western blot and normalized to those of beta -actin. The expression level of each protein is quantified by signal intensity and is indicated at the bottom of each lane. The quantitative analysis of western blot for three independent experiments was shown in S3 Fig. ANOVA with Tukey’s test was used to compare the overall difference between the groups. P < 0.05 was considered statistically significant. (B) Cells were transfected with AP-1-Luc reporter and incubated with LPS (10 μg/ml) for another 2 h. Cell lysates were subjected to luciferase activity assays to determine AP-1 luciferase activity. The results represented mean and standard deviation values from three independent experiments. *, P < 0.05 compared between two groups. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26646664), licensed under a CC-BY license. Not internally tested by R&D Systems.

JNK/c-Jun pathway is involved in LPS-mediated up-regulation of BPIFA1 expression in nasal epithelial cells.(A) Cells were pretreated for 1 h with 20 μM PD98059 (ERK inhibitor), 10 μM SP600125 (JNK inhibitor), or 20 μM SB203580 (p38 inhibitor) and incubated with 10 μg/ml LPS for 2 h. (B) Cells were transfected with a JNK-dominant negative (DN-JNK) mutant for 24 h or pretreated with SP600125 for 30 min prior to incubation with LPS for 1 h. The protein expression levels were determined using western blot. beta -actin was used as the loading control. The western blots were carried out independently in triplicate and results were representative of one of three independent experiments. The expression level of each protein was quantified by signal intensity and was indicated at the bottom of each lane. The quantitative analysis of western blot for three independent experiments was shown in S2 Fig. ANOVA with Tukey’s test was used to compare the overall difference between the groups. P < 0.05 was considered statistically significant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26646664), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB
• Clone: 251512
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PLUNC Antibody (251512) Summary

• Immunogen: E. coli-derived recombinant human PLUNC
  Gln20-Val256
  Accession # Q9NP55
• Specificity: Detects human PLUNC in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: BPIFA1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PLUNC Antibody (251512)

• bA49G10.5
• BPIFA1
• ligand-binding protein RYA3
• LPLUNC3
• Lung-specific protein X
• LUNX
• LUNXNASG
• Nasopharyngeal carcinoma-related protein
• Palate lung and nasal epithelium clone protein
• palate, lung and nasal epithelium associated
• palate, lung and nasal epithelium carcinoma associated
• PLUNC
• protein Plunc
• Secretory protein in upper respiratory tracts
• SPLUNC1
• SPLUNC1SPURT
• tracheal epithelium enriched protein
• Tracheal epithelium-enriched protein
• Von Ebner protein Hl

Background

PLUNC, also named SPLUNC1, is a secreted protein that is expressed in the secretory ducts and submucosal glands of tracheobronchial tissues. It is structurally related to bactericidal/permeability-increasing protein (BPI) and lipopolysaccaride binding protein (LBP), which are central to the host defense against gram-negative bacteria. PLUNC belongs to the short subfamily of PLUNC family proteins and has homology only to the N-terminal domains of BPI. PLUNC may function in the innate immune response against bacteria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PLUNC Antibody (MAB1897)(8)

Publications using MAB1897

• Briana R Dye, Priya H Dedhia, Alyssa J Miller, Melinda S Nagy, Eric S White, Lonnie D Shea et al. A bioengineered niche promotes in vivo engraftment and maturation of pluripotent stem cell derived human lung organoids eLife 2016-09-28 [PMID: 27677847] (Immunohistochemistry, Human)
• Yung-An Tsou, Chia-Der Lin, Hui-Chen Chen, Hui-Ying Hsu, Lii-Tzu Wu, Chuan Chiang-Ni et al. Interleukin-13 Inhibits Lipopolysaccharide-Induced BPIFA1 Expression in Nasal Epithelial Cells PLOS ONE 12/8/2015 [PMID: 26646664]
• Jennifer A. Bartlett, David K. Meyerholz, Christine L. Wohlford-Lenane, Paul W. Naumann, Nita H. Salzman, Paul B. McCray Increased susceptibility to otitis media in a Splunc1-deficient mouse model Disease Models & Mechanisms 5/1/2015 [PMID: 25765466]
• Liu Y, Bartlett J, Di M, Bomberger J, Chan Y, Gakhar L, Mallampalli R, McCray P, Di Y SPLUNC1/BPIFA1 contributes to pulmonary host defense against Klebsiella pneumoniae respiratory infection. Am J Pathol, 2013-03-15;182(5):1519-31. 2013-03-15 [PMID: 23499554] (Western Blot, Human)
• Pezzulo AA, Tang XX, Hoegger MJ, Alaiwa MH, Ramachandran S, Moninger TO, Karp PH, Wohlford-Lenane CL, Haagsman HP, van Eijk M, Banfi B, Horswill AR, Stoltz DA, McCray PB, Welsh MJ, Zabner J Reduced airway surface pH impairs bacterial killing in the porcine cystic fibrosis lung. Nature, 2012-07-04;487(7405):109-13. 2012-07-04 [PMID: 22763554] (Western Blot, Porcine)
• Karl G. Kohlgraf, Abbey R. Ackermann, Kindra K. Burnell, Rupasree N. Srikantha, Sophie A. Joly, Jennifer A. Bartlett et al. Quantitation of SPLUNC1 in saliva with an xMAP particle-based antibody capture and detection immunoassay Archives of Oral Biology 2/1/2012 [PMID: 21925642]
• McGillivary G, Bakaletz LO The multifunctional host defense peptide SPLUNC1 is critical for homeostasis of the mammalian upper airway. PLoS ONE, 2010-10-07;5(10):e13224. 2010-10-07 [PMID: 20949060] (Western Blot, Chinchilla)
• Steiling K, Kadar AY, Bergerat A Comparison of proteomic and transcriptomic profiles in the bronchial airway epithelium of current and never smokers. PLoS ONE, 2009-04-09;4(4):e5043. 2009-04-09 [PMID: 19357784] (Western Blot, Human)

Bioinformatics

• Gene Symbol: BPIFA1
• Uniprot:
  • Q9NP55()