PLS1 Antibody - BSA Free

Immunohistochemistry-Paraffin: PLS1 Antibody [NBP2-30592] - Staining of human small intestine shows high expression.

Immunohistochemistry-Paraffin: PLS1 Antibody [NBP2-30592] - Staining of human liver shows low expression as expected.

Immunohistochemistry-Paraffin: PLS1 Antibody [NBP2-30592] - Staining in human small intestine and liver tissues using anti-PLS1 antibody. Corresponding PLS1 RNA-seq data are presented for the same tissues.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PLS1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PLS1 Antibody - BSA Free (NBP2-30592) is a polyclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: DLIDAIAPNAVRQEMIRRENLSDEDKL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PLS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  PLS1 Protein (NBP2-30592PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PLS1 Antibody - BSA Free

• fimbrin
• I plastin
• intestine specific plastin
• Intestine-specific plastin
• I-plastin
• plastin 1 (I isoform)
• plastin 1
• Plastin-1

Background

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues. Fimbrin is a 68-kD actin-bundling protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLS1
• Uniprot:
  • Q14651(Human)