PLRG1 Antibody

Western Blot: PLRG1 Antibody [NB100-853] - Mouse kidney extract. Primary antibody at 0.5 ug/ml, 1 hr incubation. Detected using chemiluminescence.

Immunohistochemistry-Paraffin: PLRG1 Antibody [NB100-853] - Paraffin embedded human prostate tissue. Antibody at 5 ug/ml. Steamed antigen retrieval with citrate buffer pH 6. AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PLRG1 Antibody Summary

• Immunogen: Peptide with sequence C-PVSWKPEIIKRKRF corresponding to C-Terminus according to NP_002660.1, NP_001188493.1.
• Specificity: This antibody is expected to recognise isoform 1 (NP_002660.1) and isoform 2 (NP_001188493.1).
• Predicted Species: Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PLRG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 3 ug/ml
• Application Notes: WB: Approx. 60 kDa band observed in mouse kidney extracts.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PLRG1 Antibody

• MGC110980
• pleiotropic regulator 1 (PRL1 homolog, Arabidopsis)
• PRL1Arabidopsis homolog)

Background

PLRG1 is a spliceosomal protein that is highly conserved across species. PLRG1 has been shown to be part of sub-spliceosomal protein complexes that are essential for pre-mRNA splicing in both yeast and humans. PLRG1 and CDC5L interact directly in vitro. This interaction is mediated by WD40 regions in PLRG1 and the C-terminal domain of CDC5L.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PLRG1 Antibody (NB100-853)(1)

Publication using NB100-853

• Ajuh P, Lamond AI. Identification of peptide inhibitors of pre-mRNA splicing derived from theessential interaction domains of CDC5L and PLRG1 Nucleic Acids Res 2003-11-01 [PMID: 14576297]

Bioinformatics

• Gene Symbol: PLRG1
• Entrez:
  • 5356(Human)
  • 53317(Mouse)
  • 60376(Rat)
• Uniprot:
  • NP_002660.(Human)