Human PLGF ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard curve. PGF (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human PGF. |
| Standard Curve Range |
15.6 to 1000 pg/mL |
| Sensitivity |
<1 pg/mL |
| Assay Type |
Sandwich ELISA |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
PGF |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Useful for quantification. |
| Publications |
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Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Kit Components
|
Components
|
- ABC diluent buffer
- Antibody diluent buffer
- Avidin-Biotin-Peroxidase Complex (ABC), dilution 1:100
- Biotinylated anti-human PLGF antibody, dilution 1:100
- Lyophilized recombinant human PLGF standard
- One plate precoated with anti-human PLGF antibody
- Sample diluent buffer
- TMB color developing agent
- TMB stop solution
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human PLGF ELISA Kit (Colorimetric)
Background
PLGF is a growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration. It belongs to the cysteine-knot superfamily. PLGF is expressed in placental tissues, colon and mammary carcinomas. It signals through the VEGFR-1/FLT1 receptor.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Publications for PLGF ELISA Kit (KA0410)(3)
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Product General Protocols
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FAQs for PLGF ELISA Kit (KA0410). (Showing 1 - 1 of 1 FAQs).
-
What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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