PLGF Antibody (37203) [Unconjugated]

Anti-PlGF neutralising antibody blocks DMOG-induced inhibition of ECFC tube formation. ECFCs were stained with calcein (Invitrogen) and grown on Matrigel®. Cells were treated with conditioned medium harvested from ECFCs treated with a DMSO (vehicle) plus isotype control IgG, b DMOG plus isotype control IgG, c DMSO plus anti-PlGF neutralising antibody or d DMOG plus anti-PlGF neutralising antibody. e At 48 h, total tube area (μm2) was quantified using NIS Elements software (Nikon). Data plotted as mean ± SD and are representative of three independent experiments. **p < 0.01; ***p < 0.001. Scale bars: 200 μm (n = 3). DMOG dimethyloxalylglycine, PlGF placental growth factor Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27899144), licensed under a CC-BY license. Not internally tested by R&D Systems.

Anti-PlGF neutralising antibody blocks DMOG-induced inhibition of ECFC tube formation. ECFCs were stained with calcein (Invitrogen) and grown on Matrigel®. Cells were treated with conditioned medium harvested from ECFCs treated with a DMSO (vehicle) plus isotype control IgG, b DMOG plus isotype control IgG, c DMSO plus anti-PlGF neutralising antibody or d DMOG plus anti-PlGF neutralising antibody. e At 48 h, total tube area (μm2) was quantified using NIS Elements software (Nikon). Data plotted as mean ± SD and are representative of three independent experiments. **p < 0.01; ***p < 0.001. Scale bars: 200 μm (n = 3). DMOG dimethyloxalylglycine, PlGF placental growth factor Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27899144), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: ELISA(Cap)
• Clone: 37203
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PLGF Antibody (37203) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PlGF
  Ala21-Arg149
  Accession # P49763
• Specificity: Detects human PlGF in ELISAs. In ELISAs, this antibody does not cross-react with recombinant human (rh) VEGF, rhPDGF‑AA, rhPDGF‑AB, or rhPDGF‑BB.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PGF
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Human PlGF Biotinylated Antibody (Catalog number BAF264)
  Standard: Recombinant Human PlGF (Catalog number 264-PG)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PLGF Antibody (37203) [Unconjugated]

• D12S1900
• PGF
• PGFL
• placenta growth factor
• placental growth factor
• placental growth factor, vascular endothelial growth factor-related protein
• PlGF
• PlGF-2
• PLGFplacental growth factor-like
• SHGC-10760

Background

Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. As a result of alternative splicing, at least two PlGF mRNAs encoding monomeric PlGF precursors containing 149 and 170 amino acid residues have been described. The expression of PlGF is not widespread but has been detected in human umbilical vein endothelial cells, placenta, choriocarcinoma cell lines, and in renal cell carcinoma associated with angiogenesis. The PlGF proteins bind with high-affinity to VEGF R1/Flt-1 but not to VEGF R2/Flk-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PLGF Antibody (MAB264)(5)

Publications using MAB264

• Hypoxia-induced responses by endothelial colony-forming cells are modulated by placental growth factor Stem Cell Res Ther, 2016-11-29;7(1):173. 2016-11-29 [PMID: 27899144] (Neutralization, Human)
• ELENA PAGANI, FEDERICA RUFFINI, GIAN CARLO ANTONINI Antonini Cappellini, ALESSANDRO SCOPPOLA, CRISTINA FORTES, PAOLO MARCHETTI et al. Placenta growth factor and neuropilin-1 collaborate in promoting melanoma aggressiveness International Journal of Oncology 4/1/2016 [PMID: 26846845]
• Park , Su-Jung, Kim , Ki-Jo, Kim , Wan-Uk, Cho , Chul-Soo Interaction of mesenchymal stem cells with fibroblast-like synoviocytes via cadherin-11 promotes angiogenesis by enhanced secretion of placental growth factor. J Immunol, 2014-02-26;192(7):3003-10. 2014-02-26 [PMID: 24574497] (Neutralization, Mouse)
• Heijink IH, Marcel Kies P, van Oosterhout AJ, Postma DS, Kauffman HF, Vellenga E Der p, IL-4, and TGF-beta cooperatively induce EGFR-dependent TARC expression in airway epithelium. Am. J. Respir. Cell Mol. Biol., 2006-10-05;36(3):351-9. 2006-10-05 [PMID: 17023689] (Neutralization, Human)
• Cianfarani F, Zambruno G, Brogelli L, Sera F, Lacal PM, Pesce M, Capogrossi MC, Failla CM, Napolitano M, Odorisio T Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential. Am. J. Pathol., 2006-10-01;169(4):1167-82. 2006-10-01 [PMID: 17003476] (Neutralization, Human)

Bioinformatics

• Gene Symbol: PGF
• Uniprot:
  • P49763()