Plectin Antibody (7A8)

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Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr
Clone
7A8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Plectin Antibody (7A8) Summary

Immunogen
Plectin purified from rat glioma C6 cells.
Epitope
Monoclonal Anti-Plectin recognizes an epitope located in the middle section on the rod domain of the plectin molecule, approximately half way between the globular end domains. The kinase C phosphorylation site was found on the same terminal segment as thi
Specificity
The inhibits the interaction of plectin with vimentin and lamin B.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PLEC
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
WB: Detects a band of approximately 300 kDa.

Reactivity Notes

Cross-reacts with Marsupials. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for Plectin Antibody (7A8)

  • EBS1
  • HD1
  • Hemidesmosomal protein 1
  • LGMD2Q
  • PCNplectin 1, intermediate filament binding protein, 500kD
  • PLEC1b
  • PLEC1EBSO
  • plectin 1, intermediate filament binding protein 500kDa
  • plectin
  • plectin-1
  • PLTNepidermolysis bullosa simplex 1 (Ogna)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PLEC
Entrez