PLC-gamma 2 Antibody (3A8B6) - BSA Free

Images

 
Western Blot: PLC-gamma 2 Antibody (3A8B6) [NBP2-52534] - Analysis using PLCG2 mouse mAb against A431 cell lysate.
Immunocytochemistry/ Immunofluorescence: PLC-gamma 2 Antibody (3A8B6) [NBP2-52534] - Immunofluorescence analysis of Hela cells using PLCG2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have ...read more
Flow Cytometry: PLC-gamma 2 Antibody (3A8B6) [NBP2-52534] - Analysis of Hela cells using PLCG2 mouse mAb (green) and negative control (red).
ELISA: PLC-gamma 2 Antibody (3A8B6) [NBP2-52534] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, CyTOF-ready
Clone
3A8B6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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Catalog# & Formulation Size Price

PLC-gamma 2 Antibody (3A8B6) - BSA Free Summary

Immunogen
Synthesized peptide of human PLCG2 (AA: phospho-Tyrosine 753 of human Phospholipase Cg2(cERDINSLpYDVSRMYV)).
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PLCG2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Western Blot 1:500-1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
148 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for PLC-gamma 2 Antibody (3A8B6) - BSA Free

  • EC 3.1.4.11
  • Phosphoinositide phospholipase C-gamma-2
  • phospholipase C gamma 2
  • phospholipase C, gamma 2 (phosphatidylinositol-specific)
  • phospholipase C, gamma 2 (phosphatidylyinositol-specific)
  • Phospholipase C-gamma-2,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2
  • Phospholipase C-IV
  • PLCG2
  • PLCgamma 2
  • PLC-gamma 2
  • PLC-gamma-2
  • PLC-IV

Background

Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups. The phospholipase C gamma-2 (PLCgammaII) is an enzyme that plays a crucial role in intracellular signal transduction pathways. This enzyme is important because of its role in the generation of second messengers following the hydrolysis of phosphatidylinositol 4,5-bisphosphate (1). PLCgammaII has been implicated in collagen-induced signal transduction in platelets and antigen-dependent signaling in B-lymphocytes. It has been suggested that tyrosine kinases activate PLCgammaII (2). It has also been shown that PLCgammaII was required for receptor activator of NF-kappaB ligand-induced (RANKL-induced) osteoclastogenesis by differentially regulating nuclear factor of activated T cells c1 (NFATc1), activator protein-1 (AP1), and NF-kappaB (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PLCG2