PLC-gamma 1 Antibody (2A2)

Western Blot: PLC-gamma 1 Antibody (2A2) [H00005335-M01] - PLCG1 monoclonal antibody (M01), clone 2A2 Analysis of PLCG1 expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: PLC-gamma 1 Antibody (2A2) [H00005335-M01] - Analysis of monoclonal antibody to PLCG1 on HeLa cell. Antibody concentration 40 ug/ml.

Proximity Ligation Assay: PLC-gamma 1 Antibody (2A2) [H00005335-M01] - Analysis of protein-protein interactions between PTK2 and PLCG1. HeLa cells were stained with anti-PTK2 rabbit purified polyclonal 1:1200 and anti-PLCG1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Proximity Ligation Assay: PLC-gamma 1 Antibody (2A2) [H00005335-M01] - Analysis of protein-protein interactions between HCK and PLCG1. Huh7 cells were stained with anti-HCK rabbit purified polyclonal 1:1200 and anti-PLCG1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Proximity Ligation Assay: PLC-gamma 1 Antibody (2A2) [H00005335-M01] - Analysis of protein-protein interactions between PDGFRB and PLCG1. Mahlavu cells were stained with anti-PDGFRB rabbit purified polyclonal 1:600 and anti-PLCG1 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Sandwich ELISA: PLC-gamma 1 Antibody (2A2) [H00005335-M01] - Detection limit for recombinant GST tagged PLCG1 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, PLA, S-ELISA
• Clone: 2A2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PLC-gamma 1 Antibody (2A2) Summary

• Immunogen: PLCG1 (NP_002651, 1192 a.a. ~ 1291 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LKNNYSEDLELASLLIKIDIFPAKQENGDLSPFSGTSLRERGSDASGQLFHGRAREGSFESRYQQPFEDFRISQEHLADHFDSRERRAPRRTRVNGDNRL
• Localization: Cytoplasmic
• Specificity: PLCG1 - phospholipase C, gamma 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLCG1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Proximity Ligation Assay
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PLC-gamma 1 Antibody (2A2)

• EC 3.1.4.11
• gamma 1 (formerly subtype 148)
• monophosphatidylinositol phosphodiesterase
• NCKAP3,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1
• phosphatidylinositol phospholipase C
• phosphoinositidase C
• phosphoinositide phospholipase C
• Phosphoinositide phospholipase C-gamma-1
• phospholipase C, gamma 1
• phospholipase C-148
• Phospholipase C-gamma-1
• Phospholipase C-II
• PLC11-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1
• PLC148
• PLC-148
• PLCG1
• PLCgamma 1
• PLC-gamma 1
• PLC-gamma-1
• PLC-II1-phosphatidyl-D-myo-inositol-4,5-bisphosphate
• PPLCA
• triphosphoinositide phosphodiesterase

Background

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PLC-gamma 1 Antibody (H00005335-M01)(2)

Publications using H00005335-M01

• Olsson F, Sarri N, Papadopoulos N et al. Effects of 1 alpha,25-dihydroxyvitamin D3 and tacalcitol on cell signaling and anchorage-independent growth in T98G and U251 glioblastoma cells Biochemistry and Biophysics Reports 2022-09-01 [PMID: 35935021]
• Liu CH, Chen TC, Chau GY et al. An analysis of protein-protein interactions in cross-talk pathways reveals CRKL as a novel prognostic marker in hepatocellular carcinoma. Mol Cell Proteomics 2013-02-08 [PMID: 23397142]

Bioinformatics

• Gene Symbol: PLCG1
• Entrez:
  • 5335(Human)
• OMIM:
  • 172420(Human)
• Uniprot:
  • NP_002651(Human)