PLC-delta 3 Antibody - BSA Free

Immunohistochemistry-Paraffin: PLC-delta 3 Antibody [NBP2-19862] - Human hepatoma, using PLCD3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: PLC-delta 3 Antibody [NBP2-19862] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLC-delta 3 antibody (NBP2-19862) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PLC-delta 3 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human PLC-delta 3. The exact sequence is proprietary.
• Localization: Membrane; Peripheral membrane protein; Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PLCD3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 89 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol (pH7)
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PLC-delta 3 Antibody - BSA Free

• EC 3.1.4.11
• KIAA1964
• MGC71172
• Phosphoinositide phospholipase C-delta-3
• phospholipase C, delta 3,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-3
• Phospholipase C-delta-3
• PLC delta3
• PLCD3
• PLCdelta 3
• PLC-delta 3
• PLC-delta-3

Background

This gene encodes a member of the phospholipase C family, which catalyze the hydrolysis of phosphatidylinositol 4,5-bisphosphate to generate the second messengers diacylglycerol and inositol 1,4,5-trisphosphate (IP3). Diacylglycerol and IP3 mediate a variety of cellular responses to extracellular stimuli by inducing protein kinase C and increasing cytosolic Ca(2+) concentrations. This enzyme localizes to the plasma membrane and requires calcium for activation. Its activity is inhibited by spermine, sphingosine, and several phospholipids. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PLCD3
• Uniprot:
  • Q8N3E9()