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MTMR4 Antibody

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Western Blot: MTMR4 Antibody [NBP2-94706] - Analysis of extracts of various cell lines, using MTMR4 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.
Western Blot: MTMR4 Antibody [NBP2-94706] - Analysis of extracts of various cell lines, using MTMR4 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per ...read more
Immunocytochemistry/ Immunofluorescence: MTMR4 Antibody [NBP2-94706] - Analysis of U-2 OS cells using MTMR4. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: MTMR4 Antibody [NBP2-94706] - Analysis of L929 cells using MTMR4. Blue: DAPI for nuclear staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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MTMR4 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 920-1195 of human MTMR4 (NP_004678.3).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MTMR4
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:2000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
Theoretical MW
133 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for MTMR4 Antibody

  • EC 3.1.3.48
  • FYVE-DSP2
  • KIAA0647zinc finger, FYVE domain containing 11
  • myotubularin related protein 4
  • ZFYVE11FYVE domain-containing dual specificity protein phosphatase 2
  • Zinc finger FYVE domain-containing protein 11

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Myotubularin
Antibody
NBP1-86113
Immunocytochemistry/Immunofluorescence: Myotubularin Antibody [NBP1-86113] - Staining of human cell line U-251 MG shows localization to plasma membrane.
Immunohistochemistry-Paraffin: Myotubularin Antibody [NBP1-86113] - Staining of human testis shows moderate to strong cytoplasmic positivity in subsets of cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Myotubularin Antibody [NBP1-86113] - Staining of human duodenum shows strong cytoplasmic and membranous positivity in glandular cells (goblet cells).
(2 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
MTMR3 Antibody
(1E11)
H00008897-M07
Western Blot: MTMR3 Antibody (1E11) [H00008897-M07] - Western Blot analysis of MTMR3 expression in transfected 293T cell line by MTMR3 monoclonal antibody (M07), clone 1E11. Lane 1: MTMR3 transfected lysate (133.6 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: MTMR3 Antibody (1E11) [H00008897-M07] - Analysis of monoclonal antibody to MTMR3 on HeLa cell. Antibody concentration 15 ug/ml
ELISA: MTMR3 Antibody (1E11.) [H00008897-M07] - MTMR3 Antibody (1E11) [H00008897-M07] - Detection limit for recombinant GST tagged MTMR3 is 0.1 ng/ml as a capture antibody.
Species: Hu
Applications: WB, ELISA, ICC/IF, S-ELISA
DSPP Antibody
NBP2-92546
Western Blot: DSPP Antibody [NBP2-92546] - Analysis of extracts of various cell lines, using DSPP at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
Immunocytochemistry/Immunofluorescence: DSPP Antibody [NBP2-92546] - Analysis of U-2 OS cells using DSPP. Blue: DAPI for nuclear staining.
Immunocytochemistry/Immunofluorescence: DSPP Antibody [NBP2-92546] - Analysis of H9C2 cells using DSPP. Blue: DAPI for nuclear staining.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
PLC-delta 3
Antibody
AF5315
Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line, 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse PLC‑δ3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5315) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for PLC‑δ3 at approximately 100‑110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu, Mu
Applications: WB
EGFR Antibody
(DH8.3) - EGFRvIII, M ...
NBP2-50599
Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.
(5 Publications)
Species: Hu
Applications: WB, ELISA, Flow, IHC, IP, CyTOF-ready
NEDD4 Antibody
NBP2-27201
Western Blot: NEDD4 Antibody [NBP2-27201] - Analysis of NEDD4 in mouse embryo brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27201. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Species: Hu, Mu, Rt, Eq, Pm
Applications: WB
TGF-beta 1
[Unconjugated]
7754-BH/CF
TGF-beta 1 [Unconjugated]
(5 Publications)
Species: Hu
ABCC11 Antibody
(4H6)
H00085320-M02
Immunocytochemistry/Immunofluorescence: ABCC11 Antibody (4H6) [H00085320-M02] - Analysis of monoclonal antibody to ABCC11 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: ABCC11 Antibody (4H6) [H00085320-M02] - Detection limit for recombinant GST tagged ABCC11 is 0.1 ng/ml as a capture antibody.
(1 Review)
(1 Publication)
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, S-ELISA
Mad Antibody
NBP1-89979
Immunohistochemistry-Paraffin: Mad Antibody [NBP1-89979] - Staining in human bone marrow and liver tissues using anti-MXD1 antibody. Corresponding MXD1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Mad Antibody [NBP1-89979] - Staining of human bone marrow shows high expression.
Immunohistochemistry-Paraffin: Mad Antibody [NBP1-89979] - Staining of human liver shows low expression as expected.
Species: Hu
Applications: IHC, IHC-P
Smad2/3 Antibody
[Unconjugated]
AF3797
Western blot shows lysates of A549 human lung carcinoma cell line, Jurkat human acute T cell leukemia cell line, HEK293 human embryonic kidney cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human Smad2 and Smad3 were included. Specific bands for Smad2 were detected at approximately 64 and 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Smad2/3/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) or control antibody (Catalog # AB‑108‑C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The c-myc promoter was detected by standard PCR.
Smad2/3 was detected in immersion fixed MCF‑7 human breast cancer cell line induced (upper panel) or non-induced (lower panel) to undergo epithelial-mesenchymal transition (EMT) using Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and, in EMT-induced cells, nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(3 Reviews)
(12 Publications)
Species: Hu, Mu
Applications: WB, Simple Western, ChIP, ICC
Smad3 Antibody
NB100-56479
Western Blot: Smad3 Antibody [NB100-56479] - Analysis in human fibroblast and HeLa whole cell lysates. Untreated lysates show a band around 55 Kda. 1:1000 dilution, 1 hour blocking in milk 5%. Image from a verified customer review.
Immunocytochemistry/Immunofluorescence: Smad3 Antibody [NB100-56479] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Smad3 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Smad3 Antibody [NB100-56479] - Human liver tissue probed with SMAD3 antibody (bottom left, right) and isotype control antibody (top left) at 10 ug/ml.
(1 Review)
(4 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P
DGK-epsilon
Antibody (670914)
MAB5125
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, L1.2 mouse pro-B cell line, L6 rat myoblast cell line, and Nb2‑11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human DGK‑ epsilon Monoclonal Antibody (Catalog # MAB5125) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK‑ epsilon at approximately 64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
DGK‑ epsilon was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human DGK‑ epsilon Monoclonal Antibody (Catalog # MAB5125) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. This application has not yet been tested in mouse or rat samples.  View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Species: Hu, Mu, Rt
Applications: WB, IHC
AMPD1 Antibody
NBP2-24509
Western Blot: AMPD1 Antibody [NBP2-24509] - Analysis of human AMPDA1 in mouse skeletal muscle lysate in the 1) absence, 2) presence of immunizing peptide and 3) rat skeletal muscle using NBP2-24509 at 1 ug/ml.
Immunohistochemistry-Paraffin: AMPD1 Antibody [NBP2-24509] - Analysis of human skeletal muscle using NBP2-24509 at 10 ug/ml.
(1 Publication)
Species: Hu, Mu, Rt, Bv, Ca, Ma-Op, Pm
Applications: WB, IHC, IHC-P
PLEKHF2 Antibody
NBP1-82265
Western Blot: PLEKHF2 Antibody [NBP1-82265] - Analysis in control (vector only transfected HEK293T lysate) and PLEKHF2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: PLEKHF2 Antibody [NBP1-82265] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
(1 Publication)
Species: Hu
Applications: WB, IHC, IHC-P
DUSP5 Antibody
(2F3)
H00001847-M04
Western Blot: DUSP5 Antibody (2F3) [H00001847-M04] - Analysis of DUSP5 expression in transfected 293T cell line by DUSP5 monoclonal antibody (M04), clone 2F3. Lane 1: DUSP5 transfected lysate (Predicted MW: 42.1 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: DUSP5 Antibody (2F3) [H00001847-M04] - Analysis of monoclonal antibody to DUSP5 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: DUSP5 Antibody (2F3) [H00001847-M04] - Western Blot analysis of DUSP5 expression in PC-12 ( Cat # L012V1 ).
(1 Publication)
Species: Hu, Rt
Applications: WB, ELISA, ICC/IF, S-ELISA
Desmoplakin
Antibody
NBP1-49879
Immunohistochemistry-Paraffin: Desmoplakin Antibody [NBP1-49879] - Paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
(2 Publications)
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
MTMR9 Antibody
NBP1-31078
Western Blot: MTMR9 Antibody [NBP1-31078] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE MTMR9 antibody, antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: MTMR9 Antibody [NBP1-31078] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: MTMR9 Antibody [NBP1-31078] - Paraffin-embedded mouse lung. MTMR9 antibody [C3], C-term diluted at 1:500.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
BIN1 Antibody
NBP2-15587
Western Blot: BIN1 Antibody [NBP2-15587] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with BIN1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: BIN1 Antibody [NBP2-15587] - BIN1 antibody detects BIN1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BIN1 stained by BIN1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.
Immunohistochemistry-Paraffin: BIN1 Antibody [NBP2-15587] - BIN1 antibody detects BIN1 protein at cytoplasm in human papillary adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human papillary adenocarcinoma. BIN1 antibody diluted at 1:500.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P

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Bioinformatics

Gene Symbol MTMR4