PLC-delta 3 Antibody

Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse PLC-d3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5315) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for PLC-d3 at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PLC-delta 3 Antibody Summary

• Immunogen: E. coli-derived recombinant human PLC‑δ3
  Lys148-Ala344
  Accession # Q8N3E9
• Specificity: Detects human and mouse PLC‑δ3 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PLCD3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PLC-delta 3 Antibody

• EC 3.1.4.11
• KIAA1964
• MGC71172
• Phosphoinositide phospholipase C-delta-3
• phospholipase C, delta 3,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-3
• Phospholipase C-delta-3
• PLC delta3
• PLCD3
• PLCdelta 3
• PLC-delta 3
• PLC-delta-3

Background

PLC-δ3 (phospholipase C-delta 3) is an 105 kDa member of the δ-type PLC isozyme family. It is expressed in corneal epithelium, platelets, cardiac and skeletal muscle. PLC-δ3 preferentially hydrolyzes membrane PIP2. This generates IP3, which induces intracellular Ca⁺⁺ release, and DAG, activating PKC. Human PLC-δ3 is 789 amino acids (aa) in length. It contains a plextrin homology domain that tethers the enzyme to the membrane (aa 63‑172), three EF-hand motifs that spatially orient the molecule (aa 182‑285), a catalytic region (aa 337‑644), and a C2 domain that secures the catalytic region to its substrate (aa 647‑752). There are multiple potential isoforms. There is an alternate start site at Met54, a 40 aa substitution for the C-terminal 248 aa, and a Leu substitution for aa 102‑109. Over aa 148‑344, human PLC-δ3 is 93% aa identical to mouse PLC-δ3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLCD3
• Uniprot:
  • Q8N3E9()